http://purl.uniprot.org/citations/3366116 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/3366116 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/3366116 | http://www.w3.org/2000/01/rdf-schema#comment | "Orthorhombic crystals of the complex formed between bovine alpha-chymotrypsin and a recombinant human mucous proteinase inhibitor (SLPI) were grown. Data to 2.3 A resolution were collected on the area-detector diffractometer FAST. The crystal structure of the complex was solved by Patterson search techniques using chymotrypsin as a search model. A cyclic procedure of modeling and crystallographic refinement enabled the determination of the SLPI structure. The current crystallographic R-value is 0.19. SLPI has a boomerang-like shape with both wings comprising two well separated domains of similar architecture. In each domain the polypeptide chain is arranged like a stretched spiral. Two internal strands form a regular beta-hairpin loop which is accompanied by two external strands linked by the proteinase binding segment. The polypeptide segment of each domain is interconnected by four disulfide bridges with a connectivity pattern hitherto unobserved. The reactive site loop of the second domain has elastase and chymotrypsin binding properties. It contains the scissile peptide bond between Leu72I and Met73I and has a similar conformation to that observed in other serine proteinase protein inhibitors. Eight residues of this loop, two of the adjacent hairpin loop, the C-terminal segment and Trp30I are in direct contact with the cognate enzyme. The binding loop of the first domain (probably with anti-trypsin activity) is disordered due to proteolytic cleavage occurring in the course of crystallization."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.org/dc/terms/identifier | "doi:10.1002/j.1460-2075.1988.tb02819.x"xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.org/dc/terms/identifier | "doi:10.1002/j.1460-2075.1988.tb02819.x"xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/author | "Huber R."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/author | "Huber R."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/author | "Gruetter M.G."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/author | "Gruetter M.G."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/author | "Bode W."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/author | "Bode W."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/author | "Fendrich G."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/author | "Fendrich G."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/pages | "345-351"xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/pages | "345-351"xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/title | "The 2.5 A X-ray crystal structure of the acid-stable proteinase inhibitor from human mucous secretions analysed in its complex with bovine alpha-chymotrypsin."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/title | "The 2.5 A X-ray crystal structure of the acid-stable proteinase inhibitor from human mucous secretions analysed in its complex with bovine alpha-chymotrypsin."xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/volume | "7"xsd:string |
http://purl.uniprot.org/citations/3366116 | http://purl.uniprot.org/core/volume | "7"xsd:string |
http://purl.uniprot.org/citations/3366116 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3366116 |
http://purl.uniprot.org/citations/3366116 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3366116 |