| http://purl.uniprot.org/citations/33682300 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33682300 | http://www.w3.org/2000/01/rdf-schema#comment | "Serotonin N-acetyltransferase (SNAT) is the key rate-limiting enzyme in melatonin biosynthesis. It mediates melatonin biosynthesis in plants by using serotonin and 5-methoxytryptamine (5-MT), but little is known of its underlying mechanisms. Herein, we present a detailed reaction mechanism of a SNAT from Oryza sativa through combined structural and molecular dynamics (MD) analysis. We report the crystal structures of plant SNAT in the apo and binary/ternary complex forms with acetyl-CoA (AcCoA), serotonin, and 5-MT. OsSNAT exhibits a unique enzymatically active dimeric fold not found in the known structures of arylalkylamine N-acetyltransferase (AANAT) family. The key residues W188, D189, D226, N220, and Y233 located around the active pocket are important in catalysis, confirmed by site-directed mutagenesis. Combined with MD simulations, we hypothesize a novel plausible catalytic mechanism in which D226 and Y233 function as catalytic base and acid during the acetyl-transfer reaction."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.org/dc/terms/identifier | "doi:10.1002/anie.202100992"xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/author | "Chen X."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/author | "Guo Y."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/author | "Liu B."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/author | "Liu X."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/author | "Xu Y."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/author | "Zhou Y."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/author | "Zhao Y."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/author | "Liao L."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/author | "Zeng Z."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/name | "Angew Chem Int Ed Engl"xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/pages | "12020-12026"xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/title | "Structural and Molecular Dynamics Analysis of Plant Serotonin N-Acetyltransferase Reveal an Acid/Base-Assisted Catalysis in Melatonin Biosynthesis."xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://purl.uniprot.org/core/volume | "60"xsd:string |
| http://purl.uniprot.org/citations/33682300 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33682300 |
| http://purl.uniprot.org/citations/33682300 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33682300 |
| http://purl.uniprot.org/uniprot/#_Q5KQI6-mappedCitation-33682300 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33682300 |
| http://purl.uniprot.org/uniprot/Q5KQI6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33682300 |