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Introduction

Myelodysplastic syndromes (MDS) are a group of heterogeneous bone marrow clonal diseases characterized by the abnormal differentiation and development of bone marrow cells. Src homology region 2 domain-containing phosphatase (SHP)-1 is an important tumor suppressor gene that regulates the signal transducer and activator of transcription (STAT) pathway.

Methods

Survival analysis was performed to evaluate the function of decitabine (5-Aza) in treating MDS patients with and without SHP-1 methylation. The effects of 5-Aza treatment on SHP-1 expression and methylation and STAT3 phosphorylation were investigated in MDS cells by methylation-specific PCR, reverse transcription PCR, and western blotting. Cell viability and apoptosis were similarly evaluated by MTT assay and flow cytometry.

Results

High-risk MDS patients showed significant SHP-1 hypermethylation compared with low-risk patients, and patients with no SHP-1 methylation had longer overall survival. SHP-1 expression was significantly increased at mRNA and protein levels following 5-Aza treatment, while the phosphorylation of STAT3 protein was significantly decreased. Apoptosis increased significantly in MDS cells treated with higher doses of 5-Aza while cell viability decreased significantly.

Conclusion

SHP-1 hypermethylation was associated with poor prognosis in HR patients with MDS, suggesting it could be used as a prognostic indicator."xsd:string
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http://purl.uniprot.org/citations/33736531http://purl.uniprot.org/core/author"Han Y."xsd:string
http://purl.uniprot.org/citations/33736531http://purl.uniprot.org/core/author"Pang Y."xsd:string
http://purl.uniprot.org/citations/33736531http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/33736531http://purl.uniprot.org/core/author"Zhang X."xsd:string
http://purl.uniprot.org/citations/33736531http://purl.uniprot.org/core/author"Zhang J."xsd:string
http://purl.uniprot.org/citations/33736531http://purl.uniprot.org/core/author"Zhang H."xsd:string
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http://purl.uniprot.org/citations/33736531http://purl.uniprot.org/core/title"The role of Src homology region 2 domain-containing phosphatase-1 hypermethylation in the classification of patients with myelodysplastic syndromes and its association with signal transducer and activator of transcription 3 phosphorylation in skm-1 cells."xsd:string
http://purl.uniprot.org/citations/33736531http://purl.uniprot.org/core/volume"49"xsd:string
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