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http://purl.uniprot.org/citations/33781239http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/33781239http://www.w3.org/2000/01/rdf-schema#comment"

Background

The role of miR-23b-3p in insulin resistance (IR) remained poorly understood.

Methods

After acacetin injection, obesity-induced IR model was constructed with or without miR-23b-3p upregulation and Neuraminidase 1 (NEU1) overexpression in mice. Body weight, serum metabolite and fat percent of the mice were measured. Tests on oral glucose and insulin tolerance were performed, and inflammatory cytokines C-reactive protein (CRP), Interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and monocyte chemoattractant protein 1 (MCP1) levels were quantified with enzyme-linked immunosorbent assay (ELISA). The binding sites between miR-23b-3p and NEU1 were predicted by TargetScan, and verified using dual-luciferase reporter assay. Relative expressions were detected with quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Proportion of Treg and Th17 cells in total CD4+ T cells was detected with flow cytometry.

Results

MiR-23b-3p offset the effects of acacetin on body weight, fat percent, inflammatory cytokines levels and expressions of markers of regulatory T cells (Treg cells) and T helper 17 cells (Th17 cells), NEU1 and miR-23b-3p. NEU1 was a target of miR-23b-3p, and overexpressed NEU1 reversed the effects of upregulated miR-23b-3p on reducing Treg cells but increased body weight, fat percent and inflammatory cytokines levels, percentage of Th17 cells, and upregulated NEU1 expression.

Conclusion

Upregulation of miR-23b-3p offset the effects of acacetin on obesity-induced IR through regulating Treg/Th17 cell balance via targeting NEU1.The present findings provide a possible prevention strategy for obesity-induced IR."xsd:string
http://purl.uniprot.org/citations/33781239http://purl.org/dc/terms/identifier"doi:10.1186/s12902-021-00688-8"xsd:string
http://purl.uniprot.org/citations/33781239http://purl.uniprot.org/core/author"Liu X."xsd:string
http://purl.uniprot.org/citations/33781239http://purl.uniprot.org/core/author"Wei Y."xsd:string
http://purl.uniprot.org/citations/33781239http://purl.uniprot.org/core/author"Wang X."xsd:string
http://purl.uniprot.org/citations/33781239http://purl.uniprot.org/core/author"Jing J."xsd:string
http://purl.uniprot.org/citations/33781239http://purl.uniprot.org/core/author"Peng Z."xsd:string
http://purl.uniprot.org/citations/33781239http://purl.uniprot.org/core/date"2021"xsd:gYear
http://purl.uniprot.org/citations/33781239http://purl.uniprot.org/core/name"BMC Endocr Disord"xsd:string
http://purl.uniprot.org/citations/33781239http://purl.uniprot.org/core/pages"57"xsd:string
http://purl.uniprot.org/citations/33781239http://purl.uniprot.org/core/title"Acacetin ameliorates insulin resistance in obesity mice through regulating Treg/Th17 balance via MiR-23b-3p/NEU1 Axis."xsd:string
http://purl.uniprot.org/citations/33781239http://purl.uniprot.org/core/volume"21"xsd:string
http://purl.uniprot.org/citations/33781239http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/33781239
http://purl.uniprot.org/citations/33781239http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/33781239
http://purl.uniprot.org/uniprot/#_Q3UL64-mappedCitation-33781239http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33781239
http://purl.uniprot.org/uniprot/#_O35657-mappedCitation-33781239http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33781239
http://purl.uniprot.org/uniprot/#_Q7TSW3-mappedCitation-33781239http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33781239
http://purl.uniprot.org/uniprot/Q7TSW3http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/33781239
http://purl.uniprot.org/uniprot/O35657http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/33781239
http://purl.uniprot.org/uniprot/Q3UL64http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/33781239