| http://purl.uniprot.org/citations/33801762 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33801762 | http://www.w3.org/2000/01/rdf-schema#comment | "Origin recognition complex (ORC) binds to replication origins in eukaryotic DNAs and plays an important role in replication. Although yeast ORC is known to sequence-specifically bind to a replication origin, how human ORC recognizes a replication origin remains unknown. Previous genome-wide studies revealed that guanine (G)-rich sequences, potentially forming G-quadruplex (G4) structures, are present in most replication origins in human cells. We previously suggested that the region comprising residues 413-511 of human ORC subunit 1, hORC1413-511, binds preferentially to G-rich DNAs, which form a G4 structure in the absence of hORC1413-511. Here, we investigated the interaction of hORC1413-511 with various G-rich DNAs derived from human c-myc promoter and telomere regions. Fluorescence anisotropy revealed that hORC1413-511 binds preferentially to DNAs that have G4 structures over ones having double-stranded structures. Importantly, circular dichroism (CD) and nuclear magnetic resonance (NMR) showed that those G-rich DNAs retain the G4 structures even after binding with hORC1413-511. NMR chemical shift perturbation analyses revealed that the external G-tetrad planes of the G4 structures are the primary binding sites for hORC1413-511. The present study suggests that human ORC1 may recognize replication origins through the G4 structure."xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.org/dc/terms/identifier | "doi:10.3390/ijms22073481"xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/author | "Kondo K."xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/author | "Katahira M."xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/author | "Nagata T."xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/author | "Hoshina S."xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/author | "Waga S."xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/author | "Horinouchi H."xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/author | "Eladl A."xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/author | "Yamaoki Y."xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/name | "Int J Mol Sci"xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/pages | "3481"xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/title | "Investigation of the Interaction of Human Origin Recognition Complex Subunit 1 with G-Quadruplex DNAs of Human c-myc Promoter and Telomere Regions."xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/33801762 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33801762 |
| http://purl.uniprot.org/citations/33801762 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33801762 |
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| http://purl.uniprot.org/uniprot/#_P62807-mappedCitation-33801762 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33801762 |
| http://purl.uniprot.org/uniprot/#_P33778-mappedCitation-33801762 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33801762 |
| http://purl.uniprot.org/uniprot/#_P84243-mappedCitation-33801762 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33801762 |