| http://purl.uniprot.org/citations/33843588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33843588 | http://www.w3.org/2000/01/rdf-schema#comment | "The Roundabout (Robo) guidance receptor family induces axon repulsion in response to its ligand Slit by inducing local cytoskeletal changes; however, the link to the cytoskeleton and the nature of these cytoskeletal changes are poorly understood. Here, we show that the heteropentameric Scar/Wave Regulatory Complex (WRC), which drives Arp2/3-induced branched actin polymerization, is a direct effector of Robo signaling. Biochemical evidence shows that Slit triggers WRC recruitment to the Robo receptor's WRC-interacting receptor sequence (WIRS) motif. In Drosophila embryos, mutants of the WRC enhance Robo1-dependent midline crossing defects. Additionally, mutating Robo1's WIRS motif significantly reduces receptor activity in rescue assays in vivo, and CRISPR-Cas9 mutagenesis shows that the WIRS motif is essential for endogenous Robo1 function. Finally, axon guidance assays in mouse dorsal spinal commissural axons and gain-of-function experiments in chick embryos demonstrate that the WIRS motif is also required for Robo1 repulsion in mammals. Together, our data support an essential conserved role for the WIRS-WRC interaction in Robo1-mediated axon repulsion."xsd:string |
| http://purl.uniprot.org/citations/33843588 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.64474"xsd:string |
| http://purl.uniprot.org/citations/33843588 | http://purl.uniprot.org/core/author | "Chang C."xsd:string |
| http://purl.uniprot.org/citations/33843588 | http://purl.uniprot.org/core/author | "Kania A."xsd:string |
| http://purl.uniprot.org/citations/33843588 | http://purl.uniprot.org/core/author | "Bashaw G.J."xsd:string |
| http://purl.uniprot.org/citations/33843588 | http://purl.uniprot.org/core/author | "Gorla M."xsd:string |
| http://purl.uniprot.org/citations/33843588 | http://purl.uniprot.org/core/author | "Chaudhari K."xsd:string |
| http://purl.uniprot.org/citations/33843588 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33843588 | http://purl.uniprot.org/core/name | "Elife"xsd:string |
| http://purl.uniprot.org/citations/33843588 | http://purl.uniprot.org/core/pages | "e64474"xsd:string |
| http://purl.uniprot.org/citations/33843588 | http://purl.uniprot.org/core/title | "Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion."xsd:string |
| http://purl.uniprot.org/citations/33843588 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/33843588 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33843588 |
| http://purl.uniprot.org/citations/33843588 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33843588 |
| http://purl.uniprot.org/uniprot/#_M9NCT2-mappedCitation-33843588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33843588 |
| http://purl.uniprot.org/uniprot/#_F2FB81-mappedCitation-33843588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33843588 |
| http://purl.uniprot.org/uniprot/#_O44924-mappedCitation-33843588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33843588 |
| http://purl.uniprot.org/uniprot/#_M9PC46-mappedCitation-33843588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33843588 |
| http://purl.uniprot.org/uniprot/#_H5V8D0-mappedCitation-33843588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33843588 |
| http://purl.uniprot.org/uniprot/#_M9PCP4-mappedCitation-33843588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33843588 |
| http://purl.uniprot.org/uniprot/#_P32392-mappedCitation-33843588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33843588 |
| http://purl.uniprot.org/uniprot/#_Q5U176-mappedCitation-33843588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33843588 |
| http://purl.uniprot.org/uniprot/#_Q95SC7-mappedCitation-33843588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33843588 |
| http://purl.uniprot.org/uniprot/#_Q8MLQ0-mappedCitation-33843588 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33843588 |