| http://purl.uniprot.org/citations/33846633 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33846633 | http://www.w3.org/2000/01/rdf-schema#comment | "Efficient transcription of RNA polymerase II (Pol II) through nucleosomes requires the help of various factors. Here we show biochemically that Pol II transcription through a nucleosome is facilitated by the chromatin remodeler Chd1 and the histone chaperone FACT when the elongation factors Spt4/5 and TFIIS are present. We report cryo-EM structures of transcribing Saccharomyces cerevisiae Pol II-Spt4/5-nucleosome complexes with bound Chd1 or FACT. In the first structure, Pol II transcription exposes the proximal histone H2A-H2B dimer that is bound by Spt5. Pol II has also released the inhibitory DNA-binding region of Chd1 that is poised to pump DNA toward Pol II. In the second structure, Pol II has generated a partially unraveled nucleosome that binds FACT, which excludes Chd1 and Spt5. These results suggest that Pol II progression through a nucleosome activates Chd1, enables FACT binding and eventually triggers transfer of FACT together with histones to upstream DNA."xsd:string |
| http://purl.uniprot.org/citations/33846633 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41594-021-00578-6"xsd:string |
| http://purl.uniprot.org/citations/33846633 | http://purl.uniprot.org/core/author | "Cramer P."xsd:string |
| http://purl.uniprot.org/citations/33846633 | http://purl.uniprot.org/core/author | "Farnung L."xsd:string |
| http://purl.uniprot.org/citations/33846633 | http://purl.uniprot.org/core/author | "Engeholm M."xsd:string |
| http://purl.uniprot.org/citations/33846633 | http://purl.uniprot.org/core/author | "Ochmann M."xsd:string |
| http://purl.uniprot.org/citations/33846633 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33846633 | http://purl.uniprot.org/core/name | "Nat Struct Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/33846633 | http://purl.uniprot.org/core/pages | "382-387"xsd:string |
| http://purl.uniprot.org/citations/33846633 | http://purl.uniprot.org/core/title | "Structural basis of nucleosome transcription mediated by Chd1 and FACT."xsd:string |
| http://purl.uniprot.org/citations/33846633 | http://purl.uniprot.org/core/volume | "28"xsd:string |
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