| http://purl.uniprot.org/citations/33853759 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33853759 | http://www.w3.org/2000/01/rdf-schema#comment | "Cell-extracellular matrix (ECM) detachment is known to decrease extracellular signal-regulated kinase (ERK) signaling, an intracellular pathway that is central for control of cell behavior. How cell-ECM detachment is linked to downregulation of ERK signaling, however, is incompletely understood. We show here that focal adhesion protein Ras Suppressor 1 (RSU1) plays a critical role in cell-ECM detachment induced suppression of ERK signaling. We have identified prohibitin 2 (PHB2), a component of membrane lipid rafts, as a novel binding protein of RSU1, and mapped a major RSU1-binding site to PHB2 amino acids 150 to 206 in the C-terminal region of the PHB/SPFH (stomatin/prohibitin/flotillin/HflKC) domain. The PHB2 binding is mediated by multiple sites located in the N-terminal leucine-rich repeat region of RSU1. Depletion of PHB2 suppressed cell-ECM adhesion-induced ERK activation. Furthermore, cell-ECM detachment increased RSU1 association with membrane lipid rafts and interaction with PHB2. Finally, knockout of RSU1 or inhibition of RSU1 interaction with PHB2 by overexpression of the major RSU1-binding PHB2 fragment (amino acids 150-206) effectively suppressed the cell-ECM detachment induced downregulation of ERK signaling. Additionally, expression of venus-tagged wild-type RSU1 restored ERK signaling, while expression of venus-tagged PHB2-binding defective RSU1 mutant in which the N-terminal leucine-rich repeat region is deleted did not. Taken together, Our findings identify a novel RSU1-PHB2 signaling axis that senses cell-ECM detachment and links it to decreased ERK signaling."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.ra120.014413"xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/author | "Chen Y."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/author | "Deng Y."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/author | "Liu J."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/author | "Sun Y."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/author | "Tu Y."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/author | "Wang M."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/author | "Zhao H."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/author | "Zhao Z."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/author | "Wu C."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/author | "Qu J."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/author | "Chen K."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/pages | "100109"xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/title | "RSU-1 interaction with prohibitin-2 links cell-extracellular matrix detachment to downregulation of ERK signaling."xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://purl.uniprot.org/core/volume | "296"xsd:string |
| http://purl.uniprot.org/citations/33853759 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33853759 |
| http://purl.uniprot.org/citations/33853759 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33853759 |
| http://purl.uniprot.org/uniprot/#_A0A3G2KQ93-mappedCitation-33853759 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33853759 |
| http://purl.uniprot.org/uniprot/#_B0YJ73-mappedCitation-33853759 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33853759 |
| http://purl.uniprot.org/uniprot/#_A0A976LZI3-mappedCitation-33853759 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33853759 |
| http://purl.uniprot.org/uniprot/#_B4DW05-mappedCitation-33853759 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33853759 |