| http://purl.uniprot.org/citations/33914877 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/33914877 | http://www.w3.org/2000/01/rdf-schema#comment | "A missense mutant, unc-17(e245), which affects the Caenorhabditis elegans vesicular acetylcholine transporter UNC-17, has a severe uncoordinated phenotype, allowing efficient selection of dominant suppressors that revert this phenotype to wild-type. Such selections permitted isolation of numerous suppressors after EMS (ethyl methanesulfonate) mutagenesis, leading to demonstration of delays in mutation fixation after initial EMS treatment, as has been shown in T4 bacteriophage but not previously in eukaryotes. Three strong dominant extragenic suppressor loci have been defined, all of which act specifically on allele e245, which causes a G347R mutation in UNC-17. Two of the suppressors (sup-1 and sup-8/snb-1) have previously been shown to encode synaptic proteins able to interact directly with UNC-17. We found that the remaining suppressor, sup-2, corresponds to a mutation in erd-2.1, which encodes an endoplasmic reticulum retention protein; sup-2 causes a V186E missense mutation in transmembrane helix 7 of ERD-2.1. The same missense change introduced into the redundant paralogous gene erd-2.2 also suppressed unc-17(e245). Suppression presumably occurred by compensatory charge interactions between transmembrane helices of UNC-17 and ERD-2.1 or ERD-2.2, as previously proposed in work on suppression by SUP-1(G84E) or SUP-8(I97D)/synaptobrevin. erd-2.1(V186E) homozygotes were fully viable, but erd-2.1(V186E); erd-2.2(RNAi) exhibited synthetic lethality [like erd-2.1(RNAi); erd-2.2(RNAi)], indicating that the missense change in ERD-2.1 impairs its normal function in the secretory pathway but may allow it to adopt a novel moonlighting function as an unc-17 suppressor."xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.org/dc/terms/identifier | "doi:10.1093/genetics/iyab065"xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/author | "Stroud D."xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/author | "Mullen G.P."xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/author | "Hodgkin J."xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/author | "Rand J.B."xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/author | "Gavriilidis G."xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/author | "Mathews E.A."xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/author | "Duerr J.S."xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/name | "Genetics"xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/pages | "iyab065"xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/title | "Allele-specific suppression in Caenorhabditis elegans reveals details of EMS mutagenesis and a possible moonlighting interaction between the vesicular acetylcholine transporter and ERD2 receptors."xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://purl.uniprot.org/core/volume | "218"xsd:string |
| http://purl.uniprot.org/citations/33914877 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/33914877 |
| http://purl.uniprot.org/citations/33914877 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/33914877 |
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| http://purl.uniprot.org/uniprot/#_P48583-mappedCitation-33914877 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/33914877 |
| http://purl.uniprot.org/uniprot/P34711 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33914877 |
| http://purl.uniprot.org/uniprot/P48583 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/33914877 |