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http://purl.uniprot.org/citations/33995375http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/33995375http://www.w3.org/2000/01/rdf-schema#comment"HLA-B*13:01-positive patients in Thailand can develop frequent co-trimoxazole hypersensitivity reactions. This study aimed to characterize drug-specific T cells from three co-trimoxazole hypersensitive patients presenting with either Stevens-Johnson syndrome or drug reaction with eosinophilia and systemic symptoms. Two of the patients carried the HLA allele of interest, namely HLA-B*13:01. Sulfamethoxazole and nitroso sulfamethoxazole specific T cell clones were generated from T cell lines of co-trimoxazole hypersensitive HLA-B*13:01-positive patients. Clones were characterized for antigen specificity and cross-reactivity with structurally related compounds by measuring proliferation and cytokine release. Surface marker expression was characterized via flow cytometry. Mechanistic studies were conducted to assess pathways of T cell activation in response to antigen stimulation. Peripheral blood mononuclear cells from all patients were stimulated to proliferate and secrete IFN-γ with nitroso sulfamethoxazole. All sulfamethoxazole and nitroso sulfamethoxazole specific T cell clones expressed the CD4+ phenotype and strongly secreted IL-13 as well as IFN-γ, granzyme B and IL-22. No secretion of IL-17 was observed. A number of nitroso sulfamethoxazole-specific clones cross-reacted with nitroso dapsone but not sulfamethoxazole whereas sulfamethoxazole specific clones cross-reacted with nitroso sulfamethoxazole only. The nitroso sulfamethoxazole specific clones were activated in both antigen processing-dependent and -independent manner, while sulfamethoxazole activated T cell responses via direct HLA binding. Furthermore, activation of nitroso sulfamethoxazole-specific, but not sulfamethoxazole-specific, clones was blocked with glutathione. Sulfamethoxazole and nitroso sulfamethoxazole specific T cell clones from hypersensitive patients were CD4+ which suggests that HLA-B*13:01 is not directly involved in the iatrogenic disease observed in co-trimoxazole hypersensitivity patients."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.org/dc/terms/identifier"doi:10.3389/fimmu.2021.658593"xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Pirmohamed M."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Thomson P."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Sukasem C."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Tassaneeyakul W."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Naisbitt D.J."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Klaewsongkram J."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Nakkam N."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Rerkpattanapipat T."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Pratoomwun J."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Rerknimitr P."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Jaruthamsophon K."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Tiyasirichokchai R."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Srinoulprasert Y."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/author"Jinda P."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/date"2021"xsd:gYear
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/name"Front Immunol"xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/pages"658593"xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/title"Characterization of T-Cell Responses to SMX and SMX-NO in Co-Trimoxazole Hypersensitivity Patients Expressing HLA-B*13:01."xsd:string
http://purl.uniprot.org/citations/33995375http://purl.uniprot.org/core/volume"12"xsd:string
http://purl.uniprot.org/citations/33995375http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/33995375
http://purl.uniprot.org/citations/33995375http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/33995375
http://purl.uniprot.org/uniprot/#_A0A0A7C552-mappedCitation-33995375http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/33995375
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