| http://purl.uniprot.org/citations/34031393 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34031393 | http://www.w3.org/2000/01/rdf-schema#comment | "The active zone of a presynaptic nerve terminal defines sites for neurotransmitter release. Its protein machinery may be organized through liquid-liquid phase separation, a mechanism for the formation of membrane-less subcellular compartments. Here, we show that the active zone protein Liprin-α3 rapidly and reversibly undergoes phase separation in transfected HEK293T cells. Condensate formation is triggered by Liprin-α3 PKC-phosphorylation at serine-760, and RIM and Munc13 are co-recruited into membrane-attached condensates. Phospho-specific antibodies establish phosphorylation of Liprin-α3 serine-760 in transfected cells and mouse brain tissue. In primary hippocampal neurons of newly generated Liprin-α2/α3 double knockout mice, synaptic levels of RIM and Munc13 are reduced and the pool of releasable vesicles is decreased. Re-expression of Liprin-α3 restored these presynaptic defects, while mutating the Liprin-α3 phosphorylation site to abolish phase condensation prevented this rescue. Finally, PKC activation in these neurons acutely increased RIM, Munc13 and neurotransmitter release, which depended on the presence of phosphorylatable Liprin-α3. Our findings indicate that PKC-mediated phosphorylation of Liprin-α3 triggers its phase separation and modulates active zone structure and function."xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-021-23116-w"xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/author | "Kirchhausen T."xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/author | "Wong M.Y."xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/author | "Kaeser P.S."xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/author | "Nyitrai H."xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/author | "Wang S.S.H."xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/author | "Emperador-Melero J."xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/author | "de Nola G."xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/pages | "3057"xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/title | "PKC-phosphorylation of Liprin-alpha3 triggers phase separation and controls presynaptic active zone structure."xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/34031393 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34031393 |
| http://purl.uniprot.org/citations/34031393 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34031393 |
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| http://purl.uniprot.org/uniprot/#_P60469-mappedCitation-34031393 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34031393 |
| http://purl.uniprot.org/uniprot/#_Q5DTM9-mappedCitation-34031393 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34031393 |
| http://purl.uniprot.org/uniprot/#_Q9CWF5-mappedCitation-34031393 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34031393 |