| http://purl.uniprot.org/citations/340457 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/340457 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/340457 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/340457 | http://www.w3.org/2000/01/rdf-schema#comment | "Conversion of the viral DNA of phage G4 to the duplex form provided an opportunity to isolate and determine the function of the dnaG protein, the product of a gene known to be essential for replication of the Escherichia coli chromosome. This stage of G4 DNA replication requires action of three proteins: the E. coli DNA-binding protein, the dnaG protein, and the DNA polymerase III holoenzyme. The dnaG protein has been purified approximately 25,000-fold to near-homogeneity. The native protein contains a single polypeptide of 60,000 daltons. It has been assayed for its activity on G4 DNA in three ways: (a) RNA synthesis, (b) complementation for replication of an extract of a temperature-sensitive dnaG mutant, and (c) priming of DNA replication by DNA polymerase III holoenzyme. The dnaG protein is highly specific for G4 DNA and synthesizes a unique 29-residue RNA primer to be described in the suceeding paper. Other single-stranded and duplex DNA templates are inactive. RNA primer synthesis by the dnaG protein has an apparent Km for ribonucleoside triphosphates near 10 micrometer, and a narrow optimum for Mg2+. The sharp specificity of the dnaG protein in choice of template and the utilization of either deoxyribonucleotides or ribonucleotides to produce a hybrid piece only a few residues long (as described in a succeeding paper) suggests that the dnaG protein previously named RNA polymerase by renamed primase."xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)38167-x"xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(17)38167-x"xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/author | "Rowen L."xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/author | "Rowen L."xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/author | "Kornberg A."xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/author | "Kornberg A."xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/date | "1978"xsd:gYear |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/date | "1978"xsd:gYear |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/pages | "758-764"xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/pages | "758-764"xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/title | "Primase, the dnaG protein of Escherichia coli. An enzyme which starts DNA chains."xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/title | "Primase, the dnaG protein of Escherichia coli. An enzyme which starts DNA chains."xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/volume | "253"xsd:string |
| http://purl.uniprot.org/citations/340457 | http://purl.uniprot.org/core/volume | "253"xsd:string |
| http://purl.uniprot.org/citations/340457 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/340457 |
| http://purl.uniprot.org/citations/340457 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/340457 |
| http://purl.uniprot.org/citations/340457 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/340457 |
| http://purl.uniprot.org/citations/340457 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/340457 |
| http://purl.uniprot.org/citations/340457 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/340457 |