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| http://purl.uniprot.org/citations/34171463 | http://www.w3.org/2000/01/rdf-schema#comment | "The transcription factor SOX5 is present in two distinct isoforms in both human and mouse, L-SOX5 and S-SOX5 (long and short isoforms of SOX5). Here, we identified and characterized a novel transcript of Sox5 (S-Sox5 variant) in mouse testis. eCLIP-based amplification of cDNA ends were performed to identify the potential Sox5 mRNA variant. This novel transcript shares a high similarity with the previously reported S-Sox5 in nucleotide sequence, but with a unique stretch of 5'UTR and an additional exon 9. Semi-quantitative PCR analysis revealed both S-Sox5 variant and S-Sox5 express specifically in mouse testis. Both transcripts increase significantly in mouse testis at postnatal day 21, when round spermatids appear. We further made a series of truncated Sox5 constructs and tagged them with eGFP in HeLa cells. In vitro transfection assay identified the N-terminus and the DNA-binding HMG domain are required for the nuclear localization of SOX5. Our results provides a basis for the future study to investigate the biological function of SOX5 in spermatogenesis."xsd:string |
| http://purl.uniprot.org/citations/34171463 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.gep.2021.119197"xsd:string |
| http://purl.uniprot.org/citations/34171463 | http://purl.uniprot.org/core/author | "Huang C."xsd:string |
| http://purl.uniprot.org/citations/34171463 | http://purl.uniprot.org/core/author | "Zhou J."xsd:string |
| http://purl.uniprot.org/citations/34171463 | http://purl.uniprot.org/core/author | "Zheng B."xsd:string |
| http://purl.uniprot.org/citations/34171463 | http://purl.uniprot.org/core/author | "Ye L."xsd:string |
| http://purl.uniprot.org/citations/34171463 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34171463 | http://purl.uniprot.org/core/name | "Gene Expr Patterns"xsd:string |
| http://purl.uniprot.org/citations/34171463 | http://purl.uniprot.org/core/pages | "119197"xsd:string |
| http://purl.uniprot.org/citations/34171463 | http://purl.uniprot.org/core/title | "Identification of a novel Sox5 transcript in mouse testis."xsd:string |
| http://purl.uniprot.org/citations/34171463 | http://purl.uniprot.org/core/volume | "41"xsd:string |
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