| http://purl.uniprot.org/citations/34215968 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34215968 | http://www.w3.org/2000/01/rdf-schema#comment | "Mechanosensitive ion channels (MSCs) are key molecules in the mechano-electrical transduction of arterial baroreceptors. Among them, acid-sensing ion channel 2 (ASIC2) and transient receptor potential vanilloid subfamily member 1 (TRPV1) have been studied extensively and documented to play important roles. In this study, experiments using aortic arch-aortic nerve preparations isolated from rats revealed that both ASIC2 and TRPV1 are functionally necessary, as blocking either abrogated nearly all pressure-dependent neural discharge. However, whether ASIC2 and TRPV1 work in coordination remained unclear. So we carried out cell-attached patch-clamp recordings in HEK293T cells co-expressing ASIC2 and TRPV1 and found that inhibition of ASIC2 completely blocked stretch-activated currents while inhibition of TRPV1 only partially blocked these currents. Immunofluorescence staining of aortic arch-aortic adventitia from rats showed that ASIC2 and TRPV1 are co-localized in the aortic nerve endings, and co-immunoprecipitation assays confirmed that the two proteins form a compact complex in HEK293T cells and in baroreceptors. Moreover, protein modeling analysis, exogenous co-immunoprecipitation assays, and biotin pull-down assays indicated that ASIC2 and TRPV1 interact directly. In summary, our research suggests that ASIC2 and TRPV1 form a compact complex and function synergistically in the mechano-electrical transduction of arterial baroreceptors. The model of synergism between MSCs may have important biological significance beyond ASIC2 and TRPV1."xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.org/dc/terms/identifier | "doi:10.1007/s12264-021-00737-1"xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/author | "Huang H."xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/author | "Liu P."xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/author | "Zhao H."xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/author | "Zhang S."xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/author | "Zhang C."xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/author | "Wang W."xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/author | "Yan X."xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/author | "Niu W."xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/name | "Neurosci Bull"xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/pages | "1381-1396"xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/title | "ASIC2 Synergizes with TRPV1 in the Mechano-Electrical Transduction of Arterial Baroreceptors."xsd:string |
| http://purl.uniprot.org/citations/34215968 | http://purl.uniprot.org/core/volume | "37"xsd:string |
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