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http://purl.uniprot.org/citations/34301174http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/34301174http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Numerous studies have confirmed the correlation of microRNAs (miRNAs) with human disease, yet few have explored the role of miR-135 in preeclampsia (PE). This study intends to discuss miR-135's function in inflammatory response in PE by modulating proprotein convertase subtilisin/kexin-6 (PCSK6) and NLR pyrin domain containing 3 (NLRP3).

Methods

The venous blood and placental tissues were collected from PE pregnant women and 25 normal ones. The levels of miR-135, PCSK6 and NLRP3 in placenta tissues of patients were detected. Hypoxia/reoxygenation HTR-8/SVneo and HPT-8 models were established to mimic PE in vitro, and cell proliferation, colony formation, apoptosis rate, invasion, migration and inflammation were detected through gain-of and loss-of-function assays.

Results

MiR-135 was down-regulated, and PCSK6 and NLRP3 were up-regulated in PE patients. Up-regulating miR-135 or silencing PCSK6 strengthened colony formation ability, viability, invasion and migration ability, and weakened apoptosis and inflammation of H/R-treated HTR-8/SVneo and HPT-8 cells. Inhibition of NLRP3 negated the effects of silenced PCSK6 in H/R-treated HTR-8/SVneo and HPT-8 cells.

Conclusions

Altogether, we demonstrate that up-regulated miR-135 or reduced PCSK6 attenuates inflammatory response in PE by restricting NLRP3 inflammasome, which provides novel therapy for PE treatment."xsd:string
http://purl.uniprot.org/citations/34301174http://purl.org/dc/terms/identifier"doi:10.1186/s10020-021-00335-x"xsd:string
http://purl.uniprot.org/citations/34301174http://purl.uniprot.org/core/author"Li L."xsd:string
http://purl.uniprot.org/citations/34301174http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/34301174http://purl.uniprot.org/core/author"Zhang X."xsd:string
http://purl.uniprot.org/citations/34301174http://purl.uniprot.org/core/author"Wu Z."xsd:string
http://purl.uniprot.org/citations/34301174http://purl.uniprot.org/core/author"Zhao X."xsd:string
http://purl.uniprot.org/citations/34301174http://purl.uniprot.org/core/author"Mei J."xsd:string
http://purl.uniprot.org/citations/34301174http://purl.uniprot.org/core/date"2021"xsd:gYear
http://purl.uniprot.org/citations/34301174http://purl.uniprot.org/core/name"Mol Med"xsd:string
http://purl.uniprot.org/citations/34301174http://purl.uniprot.org/core/pages"82"xsd:string
http://purl.uniprot.org/citations/34301174http://purl.uniprot.org/core/title"Up-regulation of microRNA-135 or silencing of PCSK6 attenuates inflammatory response in preeclampsia by restricting NLRP3 inflammasome."xsd:string
http://purl.uniprot.org/citations/34301174http://purl.uniprot.org/core/volume"27"xsd:string
http://purl.uniprot.org/citations/34301174http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/34301174
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