| http://purl.uniprot.org/citations/34359872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34359872 | http://www.w3.org/2000/01/rdf-schema#comment | "Ovarian cancer is the most lethal gynecological malignancy, with serous histotype as the most prevalent epithelial ovarian cancer (EOC). Peritoneal ascites is a frequent comorbidity in advanced EOC. EOC-associated ascites provide a reliable sampling source for studying lymphocytes directly from tumor environment. Herein, we carried out flow cytometry-based analysis to readdress issues on NK and T lymphocyte subsets in women with advanced EOC, additionally evaluating phenotypic modulation of their intracellular pathways involved in interleukin (IL)-2 and IL-15 signaling. Results depicted ascites as an inflammatory and immunosuppressive environment, presenting significantly (p < 0.0001) higher amounts of IL-6 and IL-10 than in the patients' blood, as well as significantly (p < 0.05) increased expression of checkpoint inhibitory receptors (programmed death protein-1, PD-1) and ectonucleotidase (CD39) on T lymphocytes. However, NK lymphocytes from EOC-associated ascites showed higher (p < 0.05) pS6 phosphorylation compared with NK from blood. Additionally, in vitro treatment of lymphocytes with IL-2 or IL-15 elicited significantly (p < 0.001) phosphorylation of the STAT5 protein in NK, CD3 and CD8 lymphocytes, both from blood and ascites. EOC-associated ascites had a significantly (p < 0.0001) higher proportion of NK CD56bright lymphocytes than blood, which, in addition, were more responsive (p < 0.05) to stimulation by IL-2 than CD56dim NK. EOC-associated ascites allow studies on lymphocyte phenotype modulation in the tumor environment, where inflammatory profile contrasts with the presence of immunosuppressive elements and development of cellular self-regulating mechanisms."xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.org/dc/terms/identifier | "doi:10.3390/cells10071702"xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/author | "Yoshida A."xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/author | "Fernandes L.G.R."xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/author | "da Silva R.F."xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/author | "Guimaraes F."xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/author | "Derchain S."xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/author | "Alves P.C.M."xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/author | "Mazzola T.N."xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/author | "Tonetti C.R."xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/author | "de Souza-Araujo C.N."xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/name | "Cells"xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/pages | "1702"xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/title | "Ovarian Cancer-Associated Ascites Have High Proportions of Cytokine-Responsive CD56bright NK Cells."xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/34359872 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34359872 |
| http://purl.uniprot.org/citations/34359872 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34359872 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DGS4-mappedCitation-34359872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34359872 |
| http://purl.uniprot.org/uniprot/#_A0A087WWD4-mappedCitation-34359872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34359872 |
| http://purl.uniprot.org/uniprot/#_B4DSQ7-mappedCitation-34359872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34359872 |
| http://purl.uniprot.org/uniprot/#_B7Z8D6-mappedCitation-34359872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34359872 |
| http://purl.uniprot.org/uniprot/#_P13591-mappedCitation-34359872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34359872 |
| http://purl.uniprot.org/uniprot/#_O00557-mappedCitation-34359872 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34359872 |