Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/34384283http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/34384283http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Wilm's tumor is a common renal malignancy in childhood with unsatisfactory prognosis. microRNA-215-5p (miR-215-5p) has been reported as a tumor-suppressive miRNA in different types of human cancers, but rarely in the Wilm's tumor. In light of this, we tried to investigate the regulatory role and underlying mechanism of miR-215-5p in the Wilm's tumor.

Methods

After sample collection and cell culture, the expression of miR-215-5p and CT10 Regulator of Kinase (CRK) was detected. Then rhabdoid tumor cell lines (formerly classified as Wilms' tumor cell lines), G401 and WT-CLS1 cells were transfected with pcDNA3.1, pcDNA3.1-CRK, sh-NC, sh-CRK, agomir NC, miR-215-5p agomir, antagomir NC or miR-215-5p antagomir to explore the function of miR-215-5p and CRK in the Wilm's tumor cell proliferation and migration. Moreover, the relationship between miR-215-5p and CRK was analyzed by dual luciferase reporter gene assay.

Results

Lowly-expressed miR-215-5p and highly-expressed CRK were observed in the Wilm's tumor tissues and cells. Transfection of pcDNA3.1-CRK or miR-215-5p antagomir could promote G401 and WT-CLS1 cell proliferation and enhance migration ability, while transfection of sh-CRK or miR-215-5p agomir led to opposite results. Additionally, miR-215-5p may bind to CRK. Moreover, transfection of pcDNA3.1-CRK in G401 and WT-CLS1 cells could partially reverse the inhibitory effect of miR-215-5p agomir on the proliferation and migration of Wilm's tumor cells.

Conclusion

Our study highlighted that miR-215-5p could suppress the proliferation and migration of Wilm's tumor cells by regulating the expression of CRK, providing new ideas for molecular targeted therapy for Wilm's tumor."xsd:string
http://purl.uniprot.org/citations/34384283http://purl.org/dc/terms/identifier"doi:10.1177/15330338211036523"xsd:string
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/author"Li W."xsd:string
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/author"Qin H."xsd:string
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/author"Xin T."xsd:string
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/author"Haisha L."xsd:string
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/author"Jiheng L."xsd:string
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/author"Lingdi L."xsd:string
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/author"Xiqiang D."xsd:string
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/date"2021"xsd:gYear
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/name"Technol Cancer Res Treat"xsd:string
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/pages"15330338211036523"xsd:string
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/title"MicroRNA-215-5p Inhibits the Proliferation and Migration of Wilm's Tumor Cells by Targeting CRK."xsd:string
http://purl.uniprot.org/citations/34384283http://purl.uniprot.org/core/volume"20"xsd:string
http://purl.uniprot.org/citations/34384283http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/34384283
http://purl.uniprot.org/citations/34384283http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/34384283
http://purl.uniprot.org/uniprot/#_A0A0S2Z3K9-mappedCitation-34384283http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/34384283
http://purl.uniprot.org/uniprot/#_A0A0S2Z3Q4-mappedCitation-34384283http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/34384283
http://purl.uniprot.org/uniprot/#_L7RT18-mappedCitation-34384283http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/34384283
http://purl.uniprot.org/uniprot/#_P46108-mappedCitation-34384283http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/34384283
http://purl.uniprot.org/uniprot/A0A0S2Z3K9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/34384283
http://purl.uniprot.org/uniprot/P46108http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/34384283
http://purl.uniprot.org/uniprot/A0A0S2Z3Q4http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/34384283
http://purl.uniprot.org/uniprot/L7RT18http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/34384283
▶