http://purl.uniprot.org/citations/34403408 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/34403408 | http://www.w3.org/2000/01/rdf-schema#comment | "Dosage compensation equalizes X-linked expression between XY males and XX females. In male fruit flies, expression levels of the X-chromosome are increased approximately two-fold to compensate for their single X chromosome. In testis, dosage compensation is thought to cease during meiosis; however, the timing and degree of the resulting transcriptional suppression is difficult to separate from global meiotic downregulation of each chromosome. To address this, we analyzed testis single-cell RNA-sequencing (scRNA-seq) data from two Drosophila melanogaster strains. We found evidence that the X chromosome is equally transcriptionally active as autosomes in somatic and pre-meiotic cells, and less transcriptionally active than autosomes in meiotic and post-meiotic cells. In cells experiencing dosage compensation, close proximity to MSL (male-specific lethal) chromatin entry sites (CES) correlates with increased X chromosome transcription. We found low or undetectable levels of germline expression of most msl genes, mle, roX1 and roX2 via scRNA-seq and RNA-FISH, and no evidence of germline nuclear roX1/2 localization. Our results suggest that, although dosage compensation occurs in somatic and pre-meiotic germ cells in Drosophila testis, there might be non-canonical factors involved in the dosage compensation mechanism. The single-cell expression patterns and enrichment statistics of detected genes can be explored interactively in our database: https://zhao.labapps.rockefeller.edu/gene-expr/."xsd:string |
http://purl.uniprot.org/citations/34403408 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pgen.1009728"xsd:string |
http://purl.uniprot.org/citations/34403408 | http://purl.uniprot.org/core/author | "Shao Z."xsd:string |
http://purl.uniprot.org/citations/34403408 | http://purl.uniprot.org/core/author | "Zhao L."xsd:string |
http://purl.uniprot.org/citations/34403408 | http://purl.uniprot.org/core/author | "Witt E."xsd:string |
http://purl.uniprot.org/citations/34403408 | http://purl.uniprot.org/core/author | "Hu C."xsd:string |
http://purl.uniprot.org/citations/34403408 | http://purl.uniprot.org/core/author | "Krause H.M."xsd:string |
http://purl.uniprot.org/citations/34403408 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
http://purl.uniprot.org/citations/34403408 | http://purl.uniprot.org/core/name | "PLoS Genet"xsd:string |
http://purl.uniprot.org/citations/34403408 | http://purl.uniprot.org/core/pages | "e1009728"xsd:string |
http://purl.uniprot.org/citations/34403408 | http://purl.uniprot.org/core/title | "Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis."xsd:string |
http://purl.uniprot.org/citations/34403408 | http://purl.uniprot.org/core/volume | "17"xsd:string |
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