| http://purl.uniprot.org/citations/34405591 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34405591 | http://www.w3.org/2000/01/rdf-schema#comment | "Obesity, type 2 diabetes, and heart disease are linked to an unhealthy diet. Sarco(endo)plasmic reticulum calcium (Ca2+ ) ATPase 2a (SERCA2a) controls cardiac function by transporting Ca2+ in cardiomyocytes. SERCA2a is altered by diet and acetylation, independently; however, it is unknown if diet alters cardiac SERCA2a acetylation. Sirtuin (SIRT) 3 is an enzyme that might preserve health under conditions of macronutrient excess by modulating metabolism via regulating deacetylation of target proteins. Our objectives were to determine if muscle-specific SIRT3 overexpression attenuates the pathological effects of high fat-high sucrose (HFHS) feeding and if HFHS feeding alters cardiac SERCA2a acetylation. We also determined if SIRT3 alters cardiac SERCA2a acetylation and regulates cardiac SERCA2a activity. C57BL/6J wild-type (WT) mice and MCK-mSIRT3-M1-Flag transgenic (SIRT3TG ) mice, overexpressing SIRT3 in cardiac and skeletal muscle, were fed a standard-diet or a HFHS-diet for 4 months. SIRT3TG and WT mice developed obesity, glucose intolerance, cardiac dysfunction, and pathological cardiac remodeling after 4 months of HFHS feeding, indicating muscle-specific SIRT3 overexpression does not attenuate the pathological effects of HFHS-feeding. Overall cardiac lysine acetylation was increased by 63% in HFHS-fed mice (p = 0.022), though HFHS feeding did not alter cardiac SERCA2a acetylation. Cardiac SERCA2a acetylation was not altered by SIRT3 overexpression, whereas SERCA2a Vmax was 21% higher in SIRT3TG (p = 0.039) than WT mice. This suggests that SIRT3 overexpression enhanced cardiac SERCA2a activity without direct SERCA2a deacetylation. Muscle-specific SIRT3 overexpression may not prevent the complications associated with an unhealthy diet in mice, but it appears to enhance SERCA2a activity in the mouse heart."xsd:string |
| http://purl.uniprot.org/citations/34405591 | http://purl.org/dc/terms/identifier | "doi:10.14814/phy2.14961"xsd:string |
| http://purl.uniprot.org/citations/34405591 | http://purl.uniprot.org/core/author | "Xiang B."xsd:string |
| http://purl.uniprot.org/citations/34405591 | http://purl.uniprot.org/core/author | "Dolinsky V.W."xsd:string |
| http://purl.uniprot.org/citations/34405591 | http://purl.uniprot.org/core/author | "Duhamel T.A."xsd:string |
| http://purl.uniprot.org/citations/34405591 | http://purl.uniprot.org/core/author | "O'Hara K.A."xsd:string |
| http://purl.uniprot.org/citations/34405591 | http://purl.uniprot.org/core/author | "Oldfield C.J."xsd:string |
| http://purl.uniprot.org/citations/34405591 | http://purl.uniprot.org/core/author | "Moffatt T.L."xsd:string |
| http://purl.uniprot.org/citations/34405591 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34405591 | http://purl.uniprot.org/core/name | "Physiol Rep"xsd:string |
| http://purl.uniprot.org/citations/34405591 | http://purl.uniprot.org/core/pages | "e14961"xsd:string |
| http://purl.uniprot.org/citations/34405591 | http://purl.uniprot.org/core/title | "Muscle-specific sirtuin 3 overexpression does not attenuate the pathological effects of high-fat/high-sucrose feeding but does enhance cardiac SERCA2a activity."xsd:string |
| http://purl.uniprot.org/citations/34405591 | http://purl.uniprot.org/core/volume | "9"xsd:string |
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