| http://purl.uniprot.org/citations/34428697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34428697 | http://www.w3.org/2000/01/rdf-schema#comment | "Rad4/XPC recognizes diverse DNA lesions to initiate nucleotide excision repair (NER). However, NER propensities among lesions vary widely and repair-resistant lesions are persistent and thus highly mutagenic. Rad4 recognizes repair-proficient lesions by unwinding ('opening') the damaged DNA site. Such 'opening' is also observed on a normal DNA sequence containing consecutive C/G's (CCC/GGG) when tethered to Rad4 to prevent protein diffusion. However, it was unknown if such tethering-facilitated DNA 'opening' could occur on any DNA or if certain structures/sequences would resist being 'opened'. Here, we report that DNA containing alternating C/G's (CGC/GCG) failed to be opened even when tethered; instead, Rad4 bound in a 180°-reversed manner, capping the DNA end. Fluorescence lifetime studies of DNA conformations in solution showed that CCC/GGG exhibits local pre-melting that is absent in CGC/GCG. In MD simulations, CGC/GCG failed to engage Rad4 to promote 'opening' contrary to CCC/GGG. Altogether, our study illustrates how local sequences can impact DNA recognition by Rad4/XPC and how certain DNA sites resist being 'opened' even with Rad4 held at that site indefinitely. The contrast between CCC/GGG and CGC/GCG sequences in Rad4-DNA recognition may help decipher a lesion's mutagenicity in various genomic sequence contexts to explain lesion-determined mutational hot and cold spots."xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.dnarep.2021.103194"xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/author | "He C."xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/author | "Paul D."xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/author | "Chakraborty S."xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/author | "Dai Q."xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/author | "Ansari A."xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/author | "Mu H."xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/author | "Broyde S."xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/author | "Min J.H."xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/author | "Tavakoli A."xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/name | "DNA Repair (Amst)"xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/pages | "103194"xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/title | "Impact of DNA sequences on DNA 'opening' by the Rad4/XPC nucleotide excision repair complex."xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://purl.uniprot.org/core/volume | "107"xsd:string |
| http://purl.uniprot.org/citations/34428697 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34428697 |
| http://purl.uniprot.org/citations/34428697 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34428697 |
| http://purl.uniprot.org/uniprot/#_P14736-mappedCitation-34428697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34428697 |
| http://purl.uniprot.org/uniprot/#_P32628-mappedCitation-34428697 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34428697 |
| http://purl.uniprot.org/uniprot/P14736 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34428697 |
| http://purl.uniprot.org/uniprot/P32628 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34428697 |