http://purl.uniprot.org/citations/34453672 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/34453672 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundAutophagy process is an important defense mechanism against intracellular infection. This process plays a critical role in limiting the development of Toxoplasma gondii. This study aimed to investigate the effects of T. gondii profilin and tachyzoites on the expression of autophagy genes.Methods and resultsPMA-activated THP-1 cell line was incubated with T. gondii profilin and tachyzoites for 6 h. After RNA extraction and cDNA synthesis, the expression of Atg5, Atg7, Atg12, and LC3b was evaluated using real-time PCR. The results revealed statistically significant downregulation of Atg5 for 1.43 (P-value = 0.0062) and 4.15 (P-value = 0.0178) folds after treatment with T. gondii profilin and tachyzoites, respectively. Similar to Atg 5, Atg 12 revealed a statistically significant downregulation for profilin (1.41 fold; P-value = 0.0047) and T. gondii tachyzoites (3.25 fold; P-value = 0.011). The expression of Atg7 elevated in both T. gondii profilin (2.083 fold; P-value = 0.0087) and tachyzoites (1.64 fold; P-value = 0.206). T. gondii profilin and tachyzoites downregulated (1.04 fold; P-value = 0.0028) and upregulated (twofold; P-value = 0.091) the expression of LC3b, respectively.ConclusionsOur findings suggest that T. gondii and profilin may manipulate autophagy via preventing from the formation of Atg5-12-16L complex to facilitate replication of T. gondii and development of toxoplasmosis."xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11033-021-06667-5"xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/author | "Zali M.R."xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/author | "Baghaei K."xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/author | "Asadzadeh Aghdaei H."xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/author | "Mirjalali H."xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/author | "Nemati S."xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/author | "Mohammad Rahimi H."xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/author | "Shahrokh S."xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/author | "Pazoki H."xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/name | "Mol Biol Rep"xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/pages | "7041-7047"xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/title | "Toxoplasma gondii profilin and tachyzoites RH strain may manipulate autophagy via downregulating Atg5 and Atg12 and upregulating Atg7."xsd:string |
http://purl.uniprot.org/citations/34453672 | http://purl.uniprot.org/core/volume | "48"xsd:string |
http://purl.uniprot.org/citations/34453672 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34453672 |
http://purl.uniprot.org/citations/34453672 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34453672 |
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