| http://purl.uniprot.org/citations/34556860 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34556860 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34556860 | http://www.w3.org/2000/01/rdf-schema#comment | "Epitranscriptomic RNA modifications can regulate RNA activity; however, there remains a major gap in our understanding of the RNA chemistry present in biological systems. Here we develop RNA-mediated activity-based protein profiling (RNABPP), a chemoproteomic strategy that relies on metabolic RNA labeling, mRNA interactome capture and quantitative proteomics, to investigate RNA-modifying enzymes in human cells. RNABPP with 5-fluoropyrimidines allowed us to profile 5-methylcytidine (m5C) and 5-methyluridine (m5U) methyltransferases. Further, we uncover a new mechanism-based crosslink between 5-fluorouridine (5-FUrd)-modified RNA and the dihydrouridine synthase (DUS) homolog DUS3L. We investigate the mechanism of crosslinking and use quantitative nucleoside liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis and 5-FUrd-based crosslinking and immunoprecipitation (CLIP) sequencing to map DUS3L-dependent dihydrouridine (DHU) modifications across the transcriptome. Finally, we show that DUS3L-knockout (KO) cells have compromised protein translation rates and impaired cellular proliferation. Taken together, our work provides a general approach for profiling RNA-modifying enzyme activity in living cells and reveals new pathways for epitranscriptomic RNA regulation."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41589-021-00874-8"xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41589-021-00874-8"xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Li A."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Li A."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Nguyen T."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Nguyen T."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Dai W."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Dai W."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Kleiner R.E."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Kleiner R.E."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Leach R.W."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Leach R.W."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Wuehr M."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Wuehr M."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Yu N.J."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/author | "Yu N.J."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/name | "Nat. Chem. Biol."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/name | "Nat. Chem. Biol."xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/pages | "1178-1187"xsd:string |
| http://purl.uniprot.org/citations/34556860 | http://purl.uniprot.org/core/pages | "1178-1187"xsd:string |