| http://purl.uniprot.org/citations/34646383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34646383 | http://www.w3.org/2000/01/rdf-schema#comment | "Rationale: Regulatory T cells (Treg cells) play an important role in maintaining peripheral tolerance by suppressing over-activation of effector T cells. The kinase PDK1 plays a pivotal role in conventional T cell development. However, whether PDK1 signaling affects the homeostasis and function of Treg cells remains elusive. Methods: In order to evaluate the role of PDK1 in Treg cells from a genetic perspective, mice carrying the floxed PDK1 allele were crossbred with Foxp3Cre mice to efficiently deleted PDK1 in Foxp3+ Treg cells. Flow cytometry was used to detect the immune cell homeostasis of WT and PDK1fl/flFoxp3Cre mice. RNA-seq was used to assess the differences in transcriptional expression profile of WT and PDK1-deficient Treg cells. The metabolic profiles of WT and PDK1-deficient Treg cells were tested using the Glycolysis Stress Test and Mito Stress Test Kits by the Seahorse XFe96 Analyser. Results: PDK1 was essential for the establishment and maintenance of Treg cell homeostasis and function. Disruption of PDK1 in Treg cells led to a spontaneous fatal systemic autoimmune disorder and multi-tissue inflammatory damage, accompanied by a reduction in the number and function of Treg cells. The deletion of PDK1 in Treg cells destroyed the iron ion balance through regulating MEK-ERK signaling and CD71 expression, resulting in excessive production of intracellular ROS, which did not depend on the down-regulation of mTORC1 signaling. Inhibition of excessive ROS, activated MEK-Erk signaling or overload Fe2+ could partially rescue the survival of PDK1-deficient Treg cells. Conclusion: Our results defined a key finding on the mechanism by which PDK1 regulates Treg cell survival via controlling redox homeostasis."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.org/dc/terms/identifier | "doi:10.7150/thno.63992"xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Dong Z."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Li Z."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Liu F."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Luo L."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Feng P."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Guan Z."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Sun Y."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Yang Q."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Yang M."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Xiao Z."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Lv W."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/author | "Wan S."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/name | "Theranostics"xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/pages | "9503-9518"xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/title | "The kinase PDK1 regulates regulatory T cell survival via controlling redox homeostasis."xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/34646383 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34646383 |
| http://purl.uniprot.org/citations/34646383 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34646383 |
| http://purl.uniprot.org/uniprot/#_A0A3Q4EC35-mappedCitation-34646383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34646383 |
| http://purl.uniprot.org/uniprot/#_A0A3Q4EC46-mappedCitation-34646383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34646383 |
| http://purl.uniprot.org/uniprot/#_A0A991EQV1-mappedCitation-34646383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34646383 |