| http://purl.uniprot.org/citations/34662550 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34662550 | http://www.w3.org/2000/01/rdf-schema#comment | "AimsType 2 diabetes mellitus (T2DM) is a risk factor for breast cancer initiation and progression. Glutamine (GLN) is a critical nutrient for cancer cells. The aim of this study was to investigate the effect of T2DM-associated compounds upon GLN uptake by breast cancer cells.Main methodsThe in vitro uptake of 3H-GLN by breast cancer (MCF-7 and MDA-MB-231) and non-tumorigenic (MCF-12A) cell lines was measured.Key findings3H-GLN uptake in the three cell lines is mainly Na+-dependent and sensitive to the ASCT2 inhibitor GPNA. IFN-γ increased total and Na+-dependent 3H-GLN uptake in the two breast cancer cell lines, and insulin increased total and Na+-dependent 3H-GLN uptake in the non-tumorigenic cell line. GPNA abolished the increase in 3H-GLN uptake promoted by these T2DM-associated compounds. ASCT2 knockdown confirmed that the increase in 3H-GLN uptake caused by IFN-γ (in breast cancer cells) and by insulin (in non-tumorigenic cells) is ASCT2-dependent. IFN-γ (in MDA-MB-231 cells) and insulin (in MCF-12A cells) increased ASCT2 transcript and protein levels. Importantly, the pro-proliferative effect of IFN-γ in breast cancer cell lines was associated with an increase in 3H-GLN uptake which was GPNA-sensitive, blocked by ASCT2 knockdown and mediated by activation of the PI3K-, STAT3- and STAT1 intracellular signalling pathways.SignificanceIFN-γ and insulin possess pro-proliferative effects in breast cancer and non-cancer cell lines, respectively, which are dependent on an increase in ASCT2-mediated glutamine transport. Thus, an effective inhibition of ASCT2-mediated glutamine uptake may be a therapeutic strategy against human breast cancer in T2DM patients."xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.lfs.2021.120054"xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://purl.uniprot.org/core/author | "Silva C."xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://purl.uniprot.org/core/author | "Andrade N."xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://purl.uniprot.org/core/author | "Ferreira A.C."xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://purl.uniprot.org/core/author | "Martel F."xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://purl.uniprot.org/core/author | "Rodrigues I."xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://purl.uniprot.org/core/author | "Soares M.L."xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34662550 | http://purl.uniprot.org/core/name | "Life Sci"xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://purl.uniprot.org/core/pages | "120054"xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://purl.uniprot.org/core/title | "The pro-proliferative effect of interferon-gamma in breast cancer cell lines is dependent on stimulation of ASCT2-mediated glutamine cellular uptake."xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://purl.uniprot.org/core/volume | "286"xsd:string |
| http://purl.uniprot.org/citations/34662550 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34662550 |
| http://purl.uniprot.org/citations/34662550 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34662550 |
| http://purl.uniprot.org/uniprot/#_B4DE27-mappedCitation-34662550 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34662550 |
| http://purl.uniprot.org/uniprot/#_Q15758-mappedCitation-34662550 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34662550 |
| http://purl.uniprot.org/uniprot/#_Q59ES3-mappedCitation-34662550 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34662550 |
| http://purl.uniprot.org/uniprot/#_Q71UA6-mappedCitation-34662550 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34662550 |
| http://purl.uniprot.org/uniprot/Q59ES3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34662550 |
| http://purl.uniprot.org/uniprot/B4DE27 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34662550 |
| http://purl.uniprot.org/uniprot/Q15758 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34662550 |
| http://purl.uniprot.org/uniprot/Q71UA6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34662550 |