| http://purl.uniprot.org/citations/34671025 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34671025 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34671025 | http://www.w3.org/2000/01/rdf-schema#comment | "Eukaryotic RNA polymerase I (Pol I) transcribes ribosomal DNA and generates RNA for ribosome synthesis. Pol I accounts for the majority of cellular transcription activity and dysregulation of Pol I transcription leads to cancers and ribosomopathies. Despite extensive structural studies of yeast Pol I, structure of human Pol I remains unsolved. Here we determined the structures of the human Pol I in the pre-translocation, post-translocation, and backtracked states at near-atomic resolution. The single-subunit peripheral stalk lacks contacts with the DNA-binding clamp and is more flexible than the two-subunit stalk in yeast Pol I. Compared to yeast Pol I, human Pol I possesses a more closed clamp, which makes more contacts with DNA. The Pol I structure in the post-cleavage backtracked state shows that the C-terminal zinc ribbon of RPA12 inserts into an open funnel and facilitates "dinucleotide cleavage" on mismatched DNA-RNA hybrid. Critical disease-associated mutations are mapped on Pol I regions that are involved in catalysis and complex organization. In summary, the structures provide new sights into human Pol I complex organization and efficient proofreading."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41421-021-00335-5"xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41421-021-00335-5"xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Liu W."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Liu W."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Yang H."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Yang H."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Wu Z."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Wu Z."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Xu Y."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Xu Y."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Zhao D."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Zhao D."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Chen K."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/author | "Chen K."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/name | "Cell Discov."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/name | "Cell Discov."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/pages | "97-109"xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/pages | "97-109"xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/title | "Structure of the human RNA polymerase I elongation complex."xsd:string |
| http://purl.uniprot.org/citations/34671025 | http://purl.uniprot.org/core/title | "Structure of the human RNA polymerase I elongation complex."xsd:string |