| http://purl.uniprot.org/citations/34675218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34675218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34675218 | http://www.w3.org/2000/01/rdf-schema#comment | "Termination of the RNA polymerase III (Pol III)-mediated transcription requires the conversion of an elongation complex (EC) to a pre-termination complex (PTC) on poly-deoxythymidine (dT)-containing non-template strand, a mechanism distinct from Pol I and Pol II. Here, our in vitro transcription elongation assay showed that 5-7 dT-containing DNA template led to transcription termination of Pol III, but not Pol I or Pol II. We assembled human Pol III PTC on a 7 dT-containing DNA template and determined the structure at 3.6 Å resolution. The structure reveals that poly-dT are trapped in a narrow exit tunnel formed by RPC2. A hydrophobic gate of the exit tunnel separates the bases of two connected deoxythymidines and may prevent translocation of the non-template strand. The fork loop 2 stabilizes both template and non-template strands around the transcription fork, and may further prevent strand translocation. Our study shows that the Pol III-specific exit tunnel and FL2 allow for efficient translocation of non-poly-dT sequence during transcription elongation but trap poly-dT to promote DNA retention of Pol III, revealing molecular mechanism of poly-dT-dependent transcription termination of Pol III."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-021-26402-9"xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-021-26402-9"xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Liu A."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Liu A."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Wang M."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Wang M."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Yu Z."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Yu Z."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Xu Y."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Xu Y."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Zhao D."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Zhao D."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Chen K."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Chen K."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Hou H."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/author | "Hou H."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/name | "Nat. Commun."xsd:string |
| http://purl.uniprot.org/citations/34675218 | http://purl.uniprot.org/core/name | "Nat. Commun."xsd:string |