| http://purl.uniprot.org/citations/34675220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34675220 | http://www.w3.org/2000/01/rdf-schema#comment | "Human metapneumovirus (HMPV) is a leading cause of acute lower respiratory tract illness in children and adults. Repeated infections are common and can be severe in young, elderly, and immunocompromised persons due to short-lived protective humoral immunity. In turn, few protective T cell epitopes have been identified in humans. Thus, we infected transgenic mice expressing the common human HLA MHC-I allele B*07:02 (HLA-B7) with HMPV and screened a robust library of overlapping and computationally predicted HLA-B7 binding peptides. Six HLA-B7-restricted CD8+ T cell epitopes were identified using ELISPOT screening in the F, M, and N proteins, with M195-203 (M195) eliciting the strongest responses. MHC-tetramer flow cytometric staining confirmed HLA-B7 epitope-specific CD8+ T cells migrated to lungs and spleen of HMPV-immune mice. Immunization with pooled HLA-B7-restricted peptides reduced viral titer and protected mice from virulent infection. Finally, we confirmed that CD8+ T cells from HLA-B7 positive humans also recognize the identified epitopes. These results enable identification of HMPV-specific CD8+ T cells in humans and help to inform future HMPV vaccine design."xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41598-021-00023-0"xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/author | "Ecker A."xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/author | "Williams J.V."xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/author | "Lan J."xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/author | "Joyce S."xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/author | "Erickson J.J."xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/author | "Miranda-Katz M."xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/pages | "20769"xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/title | "Novel HLA-B7-restricted human metapneumovirus epitopes enhance viral clearance in mice and are recognized by human CD8+ T cells."xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/34675220 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34675220 |
| http://purl.uniprot.org/citations/34675220 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34675220 |
| http://purl.uniprot.org/uniprot/#_A0A0A7C552-mappedCitation-34675220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34675220 |
| http://purl.uniprot.org/uniprot/#_A0A0E3DC98-mappedCitation-34675220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34675220 |
| http://purl.uniprot.org/uniprot/#_A0A0E3DCA0-mappedCitation-34675220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34675220 |
| http://purl.uniprot.org/uniprot/#_A0A0E3DCA1-mappedCitation-34675220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34675220 |
| http://purl.uniprot.org/uniprot/#_A0A068B107-mappedCitation-34675220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34675220 |
| http://purl.uniprot.org/uniprot/#_A0A068B112-mappedCitation-34675220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34675220 |
| http://purl.uniprot.org/uniprot/#_A0A068B116-mappedCitation-34675220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34675220 |
| http://purl.uniprot.org/uniprot/#_A0A068B2J5-mappedCitation-34675220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34675220 |