| http://purl.uniprot.org/citations/34758320 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34758320 | http://www.w3.org/2000/01/rdf-schema#comment | "CENP-A (centromeric protein A), a histone H3 variant, specifies centromere identity and is essential to centromere maintenance. Little is known about how protein levels of CENP-A are controlled in mammalian cells. Here, we report that the phosphorylation of CENP-A Ser68 primes the ubiquitin-proteasome-mediated proteolysis of CENP-A during mitotic phase in human cultured cells. We identify two major polyubiquitination sites that are responsible for this phosphorylation-dependent degradation. Substituting the two residues, Lys49 and Lys124, with arginines abrogates proper CENP-A degradation and results in CENP-A mislocalization to non-centromeric regions. Furthermore, we find that DCAF11 (DDB1 and CUL4 associated factor 11/WDR23) is the E3 ligase that specifically mediates the observed polyubiquitination. Deletion of DCAF11 hampers CENP-A degradation and causes its mislocalization. We conclude that the Ser68 phosphorylation plays an important role in regulating cellular CENP-A homeostasis via DCAF11-mediated degradation to prevent ectopic localization of CENP-A during the cell cycle."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.celrep.2021.109987"xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Huang L."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Ge X."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Hu M."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Hu J."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Li G."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Gao S."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Zhang X."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Xu L."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Yu Z."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Wu Q."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Zhao J."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Wang K."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Zhang M."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Gu B."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Ye S."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Xu R.M."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/author | "Le R."xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/name | "Cell Rep"xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/pages | "109987"xsd:string |
| http://purl.uniprot.org/citations/34758320 | http://purl.uniprot.org/core/title | "Phosphorylation at Ser68 facilitates DCAF11-mediated ubiquitination and degradation of CENP-A during the cell cycle."xsd:string |