| http://purl.uniprot.org/citations/34767441 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34767441 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34767441 | http://www.w3.org/2000/01/rdf-schema#comment | "Mitochondrial NADH:ubiquinone oxidoreductase (complex I) is a 1-MDa membrane protein complex with a central role in energy metabolism. Redox-driven proton translocation by complex I contributes substantially to the proton motive force that drives ATP synthase. Several structures of complex I from bacteria and mitochondria have been determined, but its catalytic mechanism has remained controversial. We here present the cryo-EM structure of complex I from Yarrowia lipolytica at 2.1-Å resolution, which reveals the positions of more than 1600 protein-bound water molecules, of which ~100 are located in putative proton translocation pathways. Another structure of the same complex under steady-state activity conditions at 3.4-Å resolution indicates conformational transitions that we associate with proton injection into the central hydrophilic axis. By combining high-resolution structural data with site-directed mutagenesis and large-scale molecular dynamic simulations, we define details of the proton translocation pathways and offer insights into the redox-coupled proton pumping mechanism of complex I."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.org/dc/terms/identifier | "doi:10.1126/sciadv.abj3221"xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.org/dc/terms/identifier | "doi:10.1126/sciadv.abj3221"xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Sharma V."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Sharma V."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Parey K."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Parey K."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Kuhlbrandt W."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Kuhlbrandt W."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Xie H."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Xie H."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Vonck J."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Vonck J."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Zickermann V."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Zickermann V."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Mills D.J."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Mills D.J."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Haapanen O."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Haapanen O."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Djurabekova A."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Djurabekova A."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Yoga E.G."xsd:string |
| http://purl.uniprot.org/citations/34767441 | http://purl.uniprot.org/core/author | "Yoga E.G."xsd:string |