| http://purl.uniprot.org/citations/34798057 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34798057 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34798057 | http://www.w3.org/2000/01/rdf-schema#comment | "The epitranscriptome has emerged as a new fundamental layer of control of gene expression. Nevertheless, the determination of the transcriptome-wide occupancy and function of RNA modifications remains challenging. Here we have developed Rho-seq, an integrated pipeline detecting a range of modifications through differential modification-dependent rhodamine labeling. Using Rho-seq, we confirm that the reduction of uridine to dihydrouridine (D) by the Dus reductase enzymes targets tRNAs in E. coli and fission yeast. We find that the D modification is also present on fission yeast mRNAs, particularly those encoding cytoskeleton-related proteins, which is supported by large-scale proteome analyses and ribosome profiling. We show that the α-tubulin encoding mRNA nda2 undergoes Dus3-dependent dihydrouridylation, which affects its translation. The absence of the modification on nda2 mRNA strongly impacts meiotic chromosome segregation, resulting in low gamete viability. Applying Rho-seq to human cells revealed that tubulin mRNA dihydrouridylation is evolutionarily conserved."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2021.11.003"xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2021.11.003"xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Sun J."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Sun J."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Tran P."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Tran P."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Wery M."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Wery M."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Rougemaille M."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Rougemaille M."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Wacheul L."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Wacheul L."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Hermand D."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Hermand D."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Lafontaine D.L.J."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Lafontaine D.L.J."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Dedon P."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Dedon P."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Ernst F.G.M."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Ernst F.G.M."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Finet O."xsd:string |
| http://purl.uniprot.org/citations/34798057 | http://purl.uniprot.org/core/author | "Finet O."xsd:string |