Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/34801490http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/34801490http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/34801490http://www.w3.org/2000/01/rdf-schema#comment"Plasmid-mediated, transmissible, tigecycline-inactivating enzyme Tet(X) has attracted considerable public attention. However, so far studies have not addressed its impact on public health and the ecosystem. Herein, we report the prevalence and molecular epidemiology of tet(X)-positive bacteria (TPB) from diverse sources, investigate the host-specificity of TPB and the transferability of tet(X). Sample collection was conducted between 2018 and 2020 in 30 provinces in China. PCR screening suggested tet(X) was prevalent among freshwater fishes (24.7%, 95% CI 19.4-30.7%), followed by chickens (23.6%, 21.2-26.2%), cattle (19.3%, 16.4-22.5%), healthy individuals (6.2%, 5.4-7.1%), and patients (0.3%, 0.0-1.1%). Soil and freshwater samples all tested negative for tet(X). A total of 289 TPB were isolated from 7516 samples (120/1181 chicken, 82/669 cattle, 68/3229 healthy individual, 17/239 freshwater fish and 2/2121 clinical samples). TPB distributed in six major families of bacteria including Moraxellaceae (n = 99, 34.3%), Flavobacteriaceae (n = 95, 32.9%), Enterobacteriaceae (n = 83, 28.7%), Pseudomonadaceae (n = 9, 3.1%), Sphingobacteriaceae (n = 2, 0.7%) and unclassified Gammaproteobacteria (n = 1, 0.3%). Diverse tet(X) genes including tet(X2), tet(X3), tet(X4), tet(X5) and tet(X6) were identified from different TPB. The tet(X)-positive bacteria were highly diverse, with ST10 complex belonging to the dominant E. coli clone. Novel hosts of tet(X) including Enterobacter hormaechei, Ignatzschineria indica and Oblitimonas alkaliphila were identified. Isolates from different families exhibited different antimicrobial resistance profiles. Co-existence of tet(X) with other resistance genes such as floR (66.8%) and carbapenemase genes (33.2%) was commonly observed. tet(X) could be transferred among E. coli isolates at frequencies from 10-4 to 10-10. Species other than E. coli failed to transfer tet(X) gene to the E. coli recipient via conjugation. Discriminant analysis of principal components analysis suggested inter-host transmission of tet(X)-positive E. coli among diverse hosts was not observed. Future studies are needed to monitor the transmission trend as well as the impact of this resistance gene in clinical infection control."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.org/dc/terms/identifier"doi:10.1016/j.scitotenv.2021.151767"xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Chen S."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Chen S."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Chen G."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Chen G."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Lu J."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Lu J."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Liu C."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Liu C."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Sun C."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Sun C."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Sun Q."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Sun Q."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Wang S."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Wang S."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Zhou H."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Zhou H."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Zhang R."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Zhang R."xsd:string
http://purl.uniprot.org/citations/34801490http://purl.uniprot.org/core/author"Wang H."xsd:string
▶