| http://purl.uniprot.org/citations/34808225 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34808225 | http://www.w3.org/2000/01/rdf-schema#comment | "MicroRNA-101-3p (miR-101-3p) is a tumour suppressor that regulates cancer proliferation and apoptotic signalling. Loss of miR-101-3p increases the expression of the Polycomb Repressive Complex 2 (PRC2) subunit enhancer of zeste homolog 2 (EZH2), resulting in alterations to the epigenome and enhanced tumorigenesis. MiR-101-3p has also been shown to modulate various aspects of cellular metabolism, however little is known about the mechanisms involved. To investigate the metabolic pathways that are regulated by miR-101-3p, we performed transcriptome and functional analyses of osteosarcoma cells transfected with miR-101-3p. We found that miR-101-3p downregulates multiple mitochondrial processes, including oxidative phosphorylation, pyruvate metabolism, the citric acid cycle and phospholipid metabolism. We also found that miR-101-3p transfection disrupts the transcription of mitochondrial DNA (mtDNA) via the downregulation of the mitochondrial transcription initiation complex proteins TFB2M and Mic60. These alterations in transcript expression disrupt mitochondrial function, with significant decreases in both basal (54%) and maximal (67%) mitochondrial respiration rates. Native gel electrophoresis revealed that this diminished respiratory capacity was associated with reduced steady-state levels of mature succinate dehydrogenase (complex II), with a corresponding reduction of complex II enzymatic activity. Furthermore, miR-101-3p transfection reduced the expression of the SDHB subunit, with a concomitant disruption of the assembly of the SDHC subunit into mature complex II. Overall, we describe a new role for miR-101-3p as a modulator of mitochondrial metabolism via its regulation of multiple mitochondrial processes, including mtDNA transcription and complex II biogenesis."xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2021.167361"xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/author | "McKenzie M."xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/author | "Kang Y."xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/author | "Stojanovski D."xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/author | "Lim S.C."xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/author | "Samuel S."xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/author | "Ziemann M."xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/author | "Gantier M."xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/author | "Sanchez I.L."xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/pages | "167361"xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/title | "MicroRNA-101-3p Modulates Mitochondrial Metabolism via the Regulation of Complex II Assembly."xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://purl.uniprot.org/core/volume | "434"xsd:string |
| http://purl.uniprot.org/citations/34808225 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34808225 |
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