| http://purl.uniprot.org/citations/34822310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34822310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34822310 | http://www.w3.org/2000/01/rdf-schema#comment | "Recognition of the intron branch site (BS) by the U2 small nuclear ribonucleoprotein (snRNP) is a critical event during spliceosome assembly. In mammals, BS sequences are poorly conserved, and unambiguous intron recognition cannot be achieved solely through a base-pairing mechanism. We isolated human 17S U2 snRNP and reconstituted in vitro its adenosine 5´-triphosphate (ATP)–dependent remodeling and binding to the pre–messenger RNA substrate. We determined a series of high-resolution (2.0 to 2.2 angstrom) structures providing snapshots of the BS selection process. The substrate-bound U2 snRNP shows that SF3B6 stabilizes the BS:U2 snRNA duplex, which could aid binding of introns with poor sequence complementarity. ATP-dependent remodeling uncoupled from substrate binding captures U2 snRNA in a conformation that competes with BS recognition, providing a selection mechanism based on branch helix stability."xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.org/dc/terms/identifier | "doi:10.1126/science.abm4245"xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.org/dc/terms/identifier | "doi:10.1126/science.abm4245"xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/author | "Galej W.P."xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/author | "Galej W.P."xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/author | "Weis F."xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/author | "Weis F."xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/author | "Razew M."xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/author | "Razew M."xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/author | "Tholen J."xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/author | "Tholen J."xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/name | "Science"xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/name | "Science"xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/pages | "50-57"xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/pages | "50-57"xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/title | "Structural basis of branch site recognition by the human spliceosome."xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/title | "Structural basis of branch site recognition by the human spliceosome."xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/volume | "375"xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://purl.uniprot.org/core/volume | "375"xsd:string |
| http://purl.uniprot.org/citations/34822310 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34822310 |
| http://purl.uniprot.org/citations/34822310 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34822310 |