| http://purl.uniprot.org/citations/34834922 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34834922 | http://www.w3.org/2000/01/rdf-schema#comment | "(1) Background: During maturation of the Hepatitis B virus, a viral polymerase inside the capsid transcribes a pre-genomic RNA into a partly double stranded DNA-genome. This is followed by envelopment with surface proteins inserted into a membrane. Envelopment is hypothetically regulated by a structural signal that reports the maturation state of the genome. NMR data suggest that such a signal can be mimicked by the binding of the detergent Triton X 100 to hydrophobic pockets in the capsid spikes. (2) Methods: We have used electron cryo-microscopy and image processing to elucidate the structural changes that are concomitant with the binding of Triton X 100. (3) Results: Our maps show that Triton X 100 binds with its hydrophobic head group inside the pocket. The hydrophilic tail delineates the outside of the spike and is coordinated via Lys-96. The binding of Triton X 100 changes the rotamer conformation of Phe-97 in helix 4, which enables a π-stacking interaction with Trp-62 in helix 3. Similar changes occur in mutants with low secretion phenotypes (P5T and L60V) and in a mutant with a pre-mature secretion phenotype (F97L). (4) Conclusion: Binding of Triton X 100 is unlikely to mimic structural maturation because mutants with different secretion phenotypes show similar structural responses."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.org/dc/terms/identifier | "doi:10.3390/v13112115"xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/author | "Bottcher B."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/author | "Rasmussen T."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/author | "Kraft C."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/author | "Makbul C."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/author | "Lappe M."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/author | "Stohr M."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/author | "Griessmann M."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/author | "Katzenberger K."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/author | "Pfarr P."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/author | "Stoffer C."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/author | "Wandinger A.M."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/name | "Viruses"xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/pages | "2115"xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/title | "Binding of a Pocket Factor to Hepatitis B Virus Capsids Changes the Rotamer Conformation of Phenylalanine 97."xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://purl.uniprot.org/core/volume | "13"xsd:string |
| http://purl.uniprot.org/citations/34834922 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34834922 |
| http://purl.uniprot.org/citations/34834922 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34834922 |
| http://purl.uniprot.org/uniprot/#_P03146-mappedCitation-34834922 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34834922 |
| http://purl.uniprot.org/uniprot/P03146 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34834922 |