| http://purl.uniprot.org/citations/34877503 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34877503 | http://www.w3.org/2000/01/rdf-schema#comment | "Bispecific antibodies (Bispecifics) demonstrate exceptional clinical potential to address some of the most complex diseases. However, Bispecific production in a single cell often requires the correct pairing of multiple polypeptide chains for desired assembly. This is a considerable hurdle that hinders the development of many immunoglobulin G (IgG)-like bispecific formats. Our approach focuses on the rational engineering of charged residues to facilitate the chain pairing of distinct heavy chains (HC). Here, we deploy structure-guided protein design to engineer charge pair mutations (CPMs) placed in the CH3-CH3' interface of the fragment crystallizable (Fc) region of an antibody (Ab) to correctly steer heavy chain pairing. When used in combination with our stable effector functionless 2 (SEFL2.2) technology, we observed high pairing efficiency without significant losses in expression yields. Furthermore, we investigate the relationship between CPMs and the sequence diversity in the parental antibodies, proposing a rational strategy to deploy these engineering technologies."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.isci.2021.103447"xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Li D."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Wang Z."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Whittington D.A."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Garces F."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Mohr C."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Estes B."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Gong D."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Sudom A."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Li V."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Riley T.P."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/author | "Shi S.D."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/name | "iScience"xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/pages | "103447"xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/title | "Next generation Fc scaffold for multispecific antibodies."xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://purl.uniprot.org/core/volume | "24"xsd:string |
| http://purl.uniprot.org/citations/34877503 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34877503 |
| http://purl.uniprot.org/citations/34877503 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34877503 |
| http://purl.uniprot.org/uniprot/#_P01857-mappedCitation-34877503 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34877503 |
| http://purl.uniprot.org/uniprot/#_P01859-mappedCitation-34877503 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34877503 |
| http://purl.uniprot.org/uniprot/P01857 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34877503 |