| http://purl.uniprot.org/citations/34896361 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34896361 | http://www.w3.org/2000/01/rdf-schema#comment | "Actin is a hallmark protein of the cytoskeleton in eukaryotic cells, affecting a range of cellular functions. Actin dynamics is regulated through a myriad of actin-binding proteins and post-translational modifications. The mammalian actin family consists of six different isoforms, which vary slightly in their N-terminal (Nt) sequences. During and after synthesis, actins undergo an intricate Nt-processing that yields mature actin isoforms. The ubiquitously expressed cytoplasmic β-actin is Nt-acetylated by N-alpha acetyltransferase 80 (NAA80) yielding the Nt-sequence Ac-DDDI-. In addition, β-actin was also reported to be Nt-arginylated by arginyltransferase 1 (ATE1) after further peptidase-mediated processing, yielding RDDI-. To characterize in detail the Nt-processing of actin, we used state-of-the-art proteomics. To estimate the relative cellular levels of Nt-modified proteoforms of actin, we employed NAA80-lacking cells, in which actin was not Nt-acetylated. We found that targeted proteomics is superior to a commercially available antibody previously used to analyze Nt-arginylation of β-actin. Significantly, despite the use of sensitive mass spectrometry-based techniques, we could not confirm the existence of the previously claimed Nt-arginylated β-actin (RDDI-) in either wildtype or NAA80-lacking cells. A very minor level of Nt-arginylation of the initially cleaved β-actin (DDDI-) could be identified, but only in NAA80-lacking cells, not in wildtype cells. We also identified small fractions of cleaved and unmodified β-actin (DDI-) as well as cleaved and Nt-acetylated β-actin (Ac-DDI-). In sum, we show that the multi-step Nt-maturation of β-actin is terminated by NAA80, which Nt-acetylates the exposed Nt-Asp residues, in the virtual absence of previously claimed Nt-arginylation."xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2021.167397"xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/author | "Arnesen T."xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/author | "Gevaert K."xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/author | "Timmerman E."xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/author | "Impens F."xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/author | "Drazic A."xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/author | "Marie M."xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/author | "Varland S."xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/author | "Kajan U."xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/pages | "167397"xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/title | "The Final Maturation State of beta-actin Involves N-terminal Acetylation by NAA80, not N-terminal Arginylation by ATE1."xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://purl.uniprot.org/core/volume | "434"xsd:string |
| http://purl.uniprot.org/citations/34896361 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34896361 |
| http://purl.uniprot.org/citations/34896361 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34896361 |
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| http://purl.uniprot.org/uniprot/#_A0A8I5KTH0-mappedCitation-34896361 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34896361 |
| http://purl.uniprot.org/uniprot/#_A0A8I5KZ24-mappedCitation-34896361 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34896361 |
| http://purl.uniprot.org/uniprot/#_B2R7P0-mappedCitation-34896361 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34896361 |
| http://purl.uniprot.org/uniprot/#_A8K9E2-mappedCitation-34896361 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34896361 |
| http://purl.uniprot.org/uniprot/#_B4E107-mappedCitation-34896361 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34896361 |