| http://purl.uniprot.org/citations/34921499 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34921499 | http://www.w3.org/2000/01/rdf-schema#comment | "The ATP-binding cassette transporter MsbA is a lipid flippase, translocating lipid A, glycolipids, and lipopolysaccharides from the inner to the outer leaflet of the inner membrane of Gram-negative bacteria. It has been used as a model system for time-resolved structural studies as several MsbA structures in different states and reconstitution systems (detergent/nanodiscs/peptidiscs) are available. However, due to the limited resolution of the available structures, detailed structural information on the bound nucleotides has remained elusive. Here, we have reconstituted MsbA in saposin A-lipoprotein nanoparticles (Salipro) and determined the structure of ADP-vanadate-bound MsbA by single-particle cryo-electron microscopy to 3.5 Å resolution. This procedure has resulted in significantly improved resolution and enabled us to model all side chains and visualise detailed ADP-vanadate interactions in the nucleotide-binding domains. The approach may be applicable to other dynamic membrane proteins."xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.org/dc/terms/identifier | "doi:10.1111/febs.16327"xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/author | "Josts I."xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/author | "Moulin M."xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/author | "Sander S."xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/author | "Tidow H."xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/author | "Haertlein M."xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/author | "Forsyth V.T."xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/author | "Traore D.A.K."xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/author | "Prevost S."xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/author | "Kehlenbeck D.M."xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/name | "FEBS J"xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/pages | "2959-2970"xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/title | "Cryo-EM structure of MsbA in saposin-lipid nanoparticles (Salipro) provides insights into nucleotide coordination."xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://purl.uniprot.org/core/volume | "289"xsd:string |
| http://purl.uniprot.org/citations/34921499 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34921499 |
| http://purl.uniprot.org/citations/34921499 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34921499 |
| http://purl.uniprot.org/uniprot/#_P60752-mappedCitation-34921499 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34921499 |
| http://purl.uniprot.org/uniprot/P60752 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34921499 |