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http://purl.uniprot.org/citations/34923162http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/34923162http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

Previously we demonstrated that the secreted Ly-6/uPAR related protein-1 (SLURP1), abundantly expressed in the corneal epithelium (CE) and secreted into the tear fluid, serves as an anti-inflammatory and anti-angiogenic molecule. Here we describe the Slurp1-null (Slurp1X-/-) mouse corneal phenotype for the first time.

Methods

We compared the 10-week-old wild type (WT) and Slurp1X-/-mouse corneal (i) histology by hematoxylin-eosin and periodic acid-Schiff's reagent staining, (ii) cell proliferation by immunostaining for Ki67, (iii) cell adhesion molecules by immunostaining for desmosomal and tight junction proteins, (iv) barrier function by fluorescein staining and (v) wound-healing by epithelial debridement. Effect of SLURP1 on cell cycle was quantified in human corneal limbal epithelial (HCLE) cells engineered to express SLURP1 (HCLE-SLURP1).

Results

WT and Slurp1X-/-corneal histology was largely comparable, other than a few loosely attached superficial cells in Slurp1X-/- corneas. Compared with the WT, Slurp1X-/-corneas displayed (i) increase in Ki67+ cells, (ii) altered expression and/or localization of tight junction proteins Tjp1 and Pard3, and desmosomal Dsp, (iii) increased superficial fragility and (iv) slower CE wound healing. HCLE-SLURP1 cells displayed (i) decrease in Ki67+ cells, (ii) increased cell number doubling time, (iii) stalling in G1-S phase transition during cell cycle, and (iv) downregulation of cyclins CCNE and CCND1/D2, cyclin-dependent kinases CDK4 and CDK6, and upregulation of CDK inhibitor p15/CDKN2B.

Conclusions

Collectively, these results elucidate that Slurp1X-/-CE cell homeostasis is altered and suggest that SLURP1 is a pro-differentiation factor that stalls G1-S transition during cell cycle progression by downregulating cyclins and upregulating p15/CDKN2B."xsd:string
http://purl.uniprot.org/citations/34923162http://purl.org/dc/terms/identifier"doi:10.1016/j.jtos.2021.12.006"xsd:string
http://purl.uniprot.org/citations/34923162http://purl.uniprot.org/core/author"Tiwari A."xsd:string
http://purl.uniprot.org/citations/34923162http://purl.uniprot.org/core/author"Swamynathan S.K."xsd:string
http://purl.uniprot.org/citations/34923162http://purl.uniprot.org/core/author"Campbell G."xsd:string
http://purl.uniprot.org/citations/34923162http://purl.uniprot.org/core/author"Swamynathan S."xsd:string
http://purl.uniprot.org/citations/34923162http://purl.uniprot.org/core/date"2022"xsd:gYear
http://purl.uniprot.org/citations/34923162http://purl.uniprot.org/core/name"Ocul Surf"xsd:string
http://purl.uniprot.org/citations/34923162http://purl.uniprot.org/core/pages"1-11"xsd:string
http://purl.uniprot.org/citations/34923162http://purl.uniprot.org/core/title"Secreted Ly-6/uPAR-related protein-1 (SLURP1) is a pro-differentiation factor that stalls G1-S transition during corneal epithelial cell cycle progression."xsd:string
http://purl.uniprot.org/citations/34923162http://purl.uniprot.org/core/volume"24"xsd:string
http://purl.uniprot.org/citations/34923162http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/34923162
http://purl.uniprot.org/citations/34923162http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/34923162
http://purl.uniprot.org/uniprot/#_Q9Z0K7-mappedCitation-34923162http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/34923162
http://purl.uniprot.org/uniprot/Q9Z0K7http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/34923162