Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/34974310http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/34974310http://www.w3.org/2000/01/rdf-schema#comment"

Background

Oogenesis is a fundamental process of human reproduction, and mitochondria play crucial roles in oocyte competence. Mitochondrial ATP-dependent Lon protease 1 (LONP1) functions as a critical protein in maintaining mitochondrial and cellular homeostasis in somatic cells. However, the essential role of LONP1 in maintaining mammalian oogenesis is far from elucidated.

Methods

Using conditional oocyte Lonp1-knockout mice, RNA sequencing (RNA-seq) and coimmunoprecipitation/liquid chromatography-mass spectrometry (Co-IP/LC-MS) technology, we analysed the functions of LONP1 in mammalian oogenesis.

Findings

Conditional knockout of Lonp1 in mouse oocytes in both the primordial and growing follicle stages impairs follicular development and causes progressive oocyte death, ovarian reserve loss, and infertility. LONP1 directly interacts with apoptosis inducing factor mitochondria-associated 1 (AIFM1), and LONP1 ablation leads to the translocation of AIFM1 from the cytoplasm to the nucleus, causing apoptosis in mouse oocytes. In addition, women with pathogenic variants of LONP1 lack large antral follicles (>10 mm) in the ovaries, are infertile and present premature ovarian insufficiency.

Interpretation

We demonstrated the function of LONP1 in regulating oocyte development and survival, and in-depth analysis of LONP1 will be crucial for elucidating the mechanisms underlying premature ovarian insufficiency.

Funding

This work was supported by grants from the National Key Research and Development Program of China (2018YFC1004701), the National Nature Science Foundation of China (82001629, 81871128, 81571391, 81401166, 82030040), the Jiangsu Province Social Development Project (BE2018602), the Jiangsu Provincial Medical Youth Talent (QNRC2016006), the Youth Program of the Natural Science Foundation of Jiangsu Province (BK20200116) and Jiangsu Province Postdoctoral Research Funding (2021K277B)."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.org/dc/terms/identifier"doi:10.1016/j.ebiom.2021.103790"xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Cao Y."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Ding L."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Hu Y."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Li C."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Liu C."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Li Z."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Li S."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Liu J."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Li G."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Sun H."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Zhou J."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Zhang Y."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Yan G."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Yang Z."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Yang Y."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Lu B."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Zhen X."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Yao B."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Fu C."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Sheng X."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Qin Y."xsd:string
http://purl.uniprot.org/citations/34974310http://purl.uniprot.org/core/author"Diao Z."xsd:string
▶