| http://purl.uniprot.org/citations/34983843 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/34983843 | http://www.w3.org/2000/01/rdf-schema#comment | "In Arabidopsis, vacuolar sorting receptor isoform 1 (VSR1) sorts 12S globulins to the protein storage vacuoles during seed development. Vacuolar sorting is mediated by specific protein-protein interactions between VSR1 and the vacuolar sorting determinant located at the C terminus (ctVSD) on the cargo proteins. Here, we determined the crystal structure of the protease-associated domain of VSR1 (VSR1-PA) in complex with the C-terminal pentapeptide (468RVAAA472) of cruciferin 1, an isoform of 12S globulins. The 468RVA470 motif forms a parallel β-sheet with the switch III residues (127TMD129) of VSR1-PA, and the 471AA472 motif docks to a cradle formed by the cargo-binding loop (95RGDCYF100), making a hydrophobic interaction with Tyr99. The C-terminal carboxyl group of the ctVSD is recognized by forming salt bridges with Arg95. The C-terminal sequences of cruciferin 1 and vicilin-like storage protein 22 were sufficient to redirect the secretory red fluorescent protein (spRFP) to the vacuoles in Arabidopsis protoplasts. Adding a proline residue to the C terminus of the ctVSD and R95M substitution of VSR1 disrupted receptor-cargo interactions in vitro and led to increased secretion of spRFP in Arabidopsis protoplasts. How VSR1-PA recognizes ctVSDs of other storage proteins was modeled. The last three residues of ctVSD prefer hydrophobic residues because they form a hydrophobic cluster with Tyr99 of VSR1-PA. Due to charge-charge interactions, conserved acidic residues, Asp129 and Glu132, around the cargo-binding site should prefer basic residues over acidic ones in the ctVSD. The structural insights gained may be useful in targeting recombinant proteins to the protein storage vacuoles in seeds."xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.2111281119"xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/author | "Chen S."xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/author | "Jiang L."xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/author | "Wong K.B."xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/author | "Wong C.K."xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/author | "Wong H.Y."xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/author | "Lo A.H."xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/author | "Lui S.N."xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/author | "Tsao H.E."xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/pages | "e2111281119"xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/title | "Structural insights into how vacuolar sorting receptors recognize the sorting determinants of seed storage proteins."xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://purl.uniprot.org/core/volume | "119"xsd:string |
| http://purl.uniprot.org/citations/34983843 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/34983843 |
| http://purl.uniprot.org/citations/34983843 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/34983843 |
| http://purl.uniprot.org/uniprot/#_A0A654FGW5-mappedCitation-34983843 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34983843 |
| http://purl.uniprot.org/uniprot/#_P15455-mappedCitation-34983843 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34983843 |
| http://purl.uniprot.org/uniprot/#_P93026-mappedCitation-34983843 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/34983843 |
| http://purl.uniprot.org/uniprot/P93026 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34983843 |
| http://purl.uniprot.org/uniprot/P15455 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34983843 |
| http://purl.uniprot.org/uniprot/A0A654FGW5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/34983843 |