Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/35038705http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/35038705http://www.w3.org/2000/01/rdf-schema#comment"

Introduction

Diabetic nephropathy (DN) is the leading cause of kidney failure worldwide. To explore the pathogenesis and effective biological target of DN is beneficial to seeking novel treatment strategies.

Objective

This study aimed to investigate the role of the lncRNA Dlx6os1/SOX6/EZH2 axis in DN progression.

Methods

PAS staining was performed to evaluate extracellular matrix accumulation; ELISA was carried out to assess the levels of urine microalbumin and blood glucose concentration; RT-qPCR was carried out to detect the levels of lncRNA Dlx6os1, TNF-α, IL-1β, IL-6, SOX6, and EZH2. Western blot was performed to assess the levels of Col-IV, FN, TGF-β1, and SOX6 proteins. RIP assay was carried out to verify the interaction between lncRNA Dlx6os1 and EZH2. ChIP-qPCR was conducted to verify the interaction between EZH2 and SOX6 promoter.

Results

Our results illustrated that lncRNA Dlx6os1 was highly expressed in DN mice and HG-induced SV40 MES13 cells. LncRNA Dlx6os1 knockdown inhibited HG-induced SV40 MES13 cell proliferation, fibrosis, and inflammatory cytokine release. LncRNA Dlx6os1 inhibited SOX6 expression by recruiting EZH2 in HG-SV40 MES13 cells, and SOX6 mediated the effects of lncRNA Dlx6os1 on proliferation, fibrosis, and inflammatory factor release of HG-induced SV40 MES13 cells.

Conclusion

LncRNA Dlx6os1 accelerates the progression of DN by epigenetically repressing SOX6 via recruiting EZH2."xsd:string
http://purl.uniprot.org/citations/35038705http://purl.org/dc/terms/identifier"doi:10.1159/000520490"xsd:string
http://purl.uniprot.org/citations/35038705http://purl.uniprot.org/core/author"Huang C."xsd:string
http://purl.uniprot.org/citations/35038705http://purl.uniprot.org/core/author"Xu C.Y."xsd:string
http://purl.uniprot.org/citations/35038705http://purl.uniprot.org/core/author"Zhu S.Y."xsd:string
http://purl.uniprot.org/citations/35038705http://purl.uniprot.org/core/author"Chen Y.X."xsd:string
http://purl.uniprot.org/citations/35038705http://purl.uniprot.org/core/author"Fang X.D."xsd:string
http://purl.uniprot.org/citations/35038705http://purl.uniprot.org/core/author"Tu W.P."xsd:string
http://purl.uniprot.org/citations/35038705http://purl.uniprot.org/core/date"2022"xsd:gYear
http://purl.uniprot.org/citations/35038705http://purl.uniprot.org/core/name"Kidney Blood Press Res"xsd:string
http://purl.uniprot.org/citations/35038705http://purl.uniprot.org/core/pages"177-184"xsd:string
http://purl.uniprot.org/citations/35038705http://purl.uniprot.org/core/title"LncRNA Dlx6os1 Accelerates Diabetic Nephropathy Progression by Epigenetically Repressing SOX6 via Recruiting EZH2."xsd:string
http://purl.uniprot.org/citations/35038705http://purl.uniprot.org/core/volume"47"xsd:string
http://purl.uniprot.org/citations/35038705http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/35038705
http://purl.uniprot.org/citations/35038705http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/35038705
http://purl.uniprot.org/uniprot/#_A0A0U1RNW8-mappedCitation-35038705http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35038705
http://purl.uniprot.org/uniprot/#_E9PUW0-mappedCitation-35038705http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35038705
http://purl.uniprot.org/uniprot/#_Q3U575-mappedCitation-35038705http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35038705
http://purl.uniprot.org/uniprot/#_Q3TZH6-mappedCitation-35038705http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35038705
http://purl.uniprot.org/uniprot/#_Q8BSS6-mappedCitation-35038705http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35038705
http://purl.uniprot.org/uniprot/#_P40645-mappedCitation-35038705http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35038705
http://purl.uniprot.org/uniprot/#_Q3V1J7-mappedCitation-35038705http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35038705
http://purl.uniprot.org/uniprot/#_Q61188-mappedCitation-35038705http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35038705
http://purl.uniprot.org/uniprot/#_Q571L5-mappedCitation-35038705http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35038705
▶