| http://purl.uniprot.org/citations/35100369 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35100369 | http://www.w3.org/2000/01/rdf-schema#comment | "Conditional expression of short hairpin RNAs with binary genetic systems is an indispensable tool for studying gene function. Addressing mechanisms underlying cell-cell communication in vivo benefits from simultaneous use of 2 independent gene expression systems. To complement the abundance of existing Gal4/UAS-based resources in Drosophila, we and others have developed LexA/LexAop-based genetic tools. Here, we describe experimental and pedagogical advances that promote the efficient conversion of Drosophila Gal4 lines to LexA lines, and the generation of LexAop-short hairpin RNA lines to suppress gene function. We developed a CRISPR/Cas9-based knock-in system to replace Gal4 coding sequences with LexA, and a LexAop-based short hairpin RNA expression vector to achieve short hairpin RNA-mediated gene silencing. We demonstrate the use of these approaches to achieve targeted genetic loss-of-function in multiple tissues. We also detail our development of secondary school curricula that enable students to create transgenic flies, thereby magnifying the production of well-characterized LexA/LexAop lines for the scientific community. The genetic tools and teaching methods presented here provide LexA/LexAop resources that complement existing resources to study intercellular communication coordinating metazoan physiology and development."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.org/dc/terms/identifier | "doi:10.1093/g3journal/jkac018"xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Fox E."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Kim S.K."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Kockel L."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Park S."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Kim E.S."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Wang E."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Bennett C."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Griffin C."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Chisholm T."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Lantz N."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Morse E."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Greenwald E."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Baena-Lopez L.A."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Rankin A.E."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Tsao D.D."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Chang K.R."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Floyd J.F."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/author | "Tay A.S.Y."xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/name | "G3 (Bethesda)"xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/pages | "jkac018"xsd:string |
| http://purl.uniprot.org/citations/35100369 | http://purl.uniprot.org/core/title | "Transgenic Drosophila lines for LexA-dependent gene and growth regulation."xsd:string |