| http://purl.uniprot.org/citations/35110533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35110533 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35110533 | http://www.w3.org/2000/01/rdf-schema#comment | "Positive feedback loops involving signaling and actin assembly factors mediate the formation and remodeling of branched actin networks in processes ranging from cell and organelle motility to mechanosensation. The Arp2/3 complex inhibitor Arpin controls the directional persistence of cell migration by interrupting a feedback loop involving Rac-WAVE-Arp2/3 complex, but Arpin's mechanism of inhibition is unknown. Here, we describe the cryo-EM structure of Arpin bound to Arp2/3 complex at 3.24-Å resolution. Unexpectedly, Arpin binds Arp2/3 complex similarly to WASP-family nucleation-promoting factors (NPFs) that activate the complex. However, whereas NPFs bind to two sites on Arp2/3 complex, on Arp2-ArpC1 and Arp3, Arpin only binds to the site on Arp3. Like NPFs, Arpin has a C-helix that binds at the barbed end of Arp3. Mutagenesis studies in vitro and in cells reveal how sequence differences within the C-helix define the molecular basis for inhibition by Arpin vs. activation by NPFs."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-022-28112-2"xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-022-28112-2"xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Boczkowska M."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Boczkowska M."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Dominguez R."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Dominguez R."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Rebowski G."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Rebowski G."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "van Eeuwen T."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "van Eeuwen T."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Fregoso F.E."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Fregoso F.E."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Gautreau A.M."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Gautreau A.M."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Simanov G."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Simanov G."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Zimmet A."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/author | "Zimmet A."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/name | "Nat. Commun."xsd:string |
| http://purl.uniprot.org/citations/35110533 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |