| http://purl.uniprot.org/citations/35116022 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35116022 | http://www.w3.org/2000/01/rdf-schema#comment | "We identified SARS-CoV-2 specific antigen epitopes by HLA-A2 binding affinity analysis and characterized their ability to activate T cells. As the pandemic continues, variations in SARS-CoV-2 virus strains have been found in many countries. In this study, we directly assess the immune response to SARS-CoV-2 epitope variants. We first predicted potential HLA-A*02:01-restricted CD8+ T-cell epitopes of SARS-CoV-2. Using the T2 cell model, HLA-A*02:01-restricted T-cell epitopes were screened for their binding affinity and ability to activate T cells. Subsequently, we examined the identified epitope variations and analyzed their impact on immune response. Here, we identified specific HLA-A2-restricted T-cell epitopes in the spike protein of SARS-CoV-2. Seven epitope peptides were confirmed to bind with HLA-A*02:01 and potentially be presented by antigen-presenting cells to induce host immune responses. Tetramers containing these peptides could interact with specific CD8+ T cells from convalescent COVID-19 patients, and one dominant epitope (n-Sp1) was defined. These epitopes could activate and generate epitope-specific T cells in vitro, and those activated T cells showed cytolytic activity toward target cells. Meanwhile, n-Sp1 epitope variant 5L>F significantly decreased the proportion of specific T-cell activation; n-Sp1 epitope 8L>V variant showed significantly reduced binding to HLA-A*02:01 and decreased proportion of n-Sp1-specific CD8+ T cell, which potentially contributes to the immune escape of SARS-CoV-2. Our data indicate that the variation of a dominant epitope will cause the deficiency of HLA-A*02:01 binding and T-cell activation, which subsequently requires the formation of a new CD8+ T-cell immune response in COVID-19 patients."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.org/dc/terms/identifier | "doi:10.3389/fimmu.2021.764949"xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Chen X."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Chen G."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Gao L."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Mao L."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Su J."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Wang Z."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Wang P."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Xie C."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Zhu G."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Yuan J."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Deng J."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Su H."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Xiao C."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Qiu C."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Zhang Z.J."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Fang E.F."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Huang L.A."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/author | "Luo O.J."xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/date | "2021"xsd:gYear |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/name | "Front Immunol"xsd:string |
| http://purl.uniprot.org/citations/35116022 | http://purl.uniprot.org/core/pages | "764949"xsd:string |