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http://purl.uniprot.org/citations/35152224http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/35152224http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Women with HIV (WWH) have an increased risk to develop recurrent cervical intraepithelial neoplasia grade 2/3 (rCIN2/3) after treatment compared with HIV-negative women. Therefore, appropriate posttreatment monitoring of WWH is important. This study evaluates the performance of ASCL1 and LHX8 methylation analysis as posttreatment monitoring test in WWH treated for CIN2/3, as alternative to cytology or human papillomavirus (HPV) as follow-up test.

Design

Prospective observational cohort study.

Methods

WWH treated for CIN2/3 by large loop excision of the transformation zone (LLETZ) (n  = 61) were invited for follow-up study visits at 1, 2.5 and 4 years after baseline. Baseline and follow-up cervical scrapes were tested for cytology, HPV and DNA methylation of ASCL1 and LHX8 genes. The performance of these strategies for the detection of rCIN2/3 was evaluated in the first follow-up cervical scrape.

Results

Thirteen (21.3%) rCIN2/3 lesions were detected within 4 years of follow-up. In women without rCIN2/3 in follow-up, methylation levels of ASCL1 and LHX8 decreased significantly after LLETZ treatment (P  = 0.02 and 0.007, respectively). In women with rCIN2/3, methylation levels remained high after LLETZ treatment. The 4-year rCIN2/3 risk was 4.9% (95% CI: 0.6-16.5) for ASCL1/LHX8-negative women, 8.1% (95% CI: 1.7-21.9) for HPV-negative women and 7.7% (95% CI: 2.1-18.5) for cytology-negative women.

Conclusion

A negative ASCL1/LHX8 methylation test in follow-up is associated with a low rCIN2/3 risk and could serve as an objective test of cure and well tolerated alternative for HPV and/or cytology screening in the posttreatment monitoring of WWH."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.org/dc/terms/identifier"doi:10.1097/qad.0000000000003197"xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"Visser C."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"Meijer C.J.L.M."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"Lukhwareni A."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"Heideman D.A.M."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"Dreyer G."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"van Zummeren M."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"Steenbergen R.D.M."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"Lissenberg-Witte B.I."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"Kremer W.W."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"Bleeker M.C.G."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"Breytenbach E."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/author"Vink F.J."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/date"2022"xsd:gYear
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/name"AIDS"xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/pages"953-961"xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/title"Posttreatment monitoring by ASCL1/LHX8 methylation analysis in women with HIV treated for cervical intraepithelial neoplasia grade 2/3."xsd:string
http://purl.uniprot.org/citations/35152224http://purl.uniprot.org/core/volume"36"xsd:string
http://purl.uniprot.org/citations/35152224http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/35152224
http://purl.uniprot.org/citations/35152224http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/35152224
http://purl.uniprot.org/uniprot/#_P50553-mappedCitation-35152224http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35152224
http://purl.uniprot.org/uniprot/#_Q9BYB7-mappedCitation-35152224http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35152224
http://purl.uniprot.org/uniprot/#_Q68G74-mappedCitation-35152224http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/35152224
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