| http://purl.uniprot.org/citations/35240058 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35240058 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35240058 | http://www.w3.org/2000/01/rdf-schema#comment | "Centrioles are eukaryotic organelles that template the formation of cilia and flagella, as well as organize the microtubule network and the mitotic spindle in animal cells. Centrioles have proximal-distal polarity and a 9-fold radial symmetry imparted by a likewise symmetrical central scaffold, the cartwheel. The spindle assembly abnormal protein 6 (SAS-6) self-assembles into 9-fold radially symmetric ring-shaped oligomers that stack via an unknown mechanism to form the cartwheel. Here, we uncover a homo-oligomerization interaction mediated by the coiled-coil domain of SAS-6. Crystallographic structures of Chlamydomonas reinhardtii SAS-6 coiled-coil complexes suggest this interaction is asymmetric, thereby imparting polarity to the cartwheel. Using a cryoelectron microscopy (cryo-EM) reconstitution assay, we demonstrate that amino acid substitutions disrupting this asymmetric association also impair SAS-6 ring stacking. Our work raises the possibility that the asymmetric interaction inherent to SAS-6 coiled-coil provides a polar element for cartwheel assembly, which may assist the establishment of the centriolar proximal-distal axis."xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2022.02.005"xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2022.02.005"xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/author | "Kantsadi A.L."xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/author | "Kantsadi A.L."xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/author | "Vakonakis I."xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/author | "Vakonakis I."xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/author | "Gonczy P."xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/author | "Gonczy P."xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/author | "Hatzopoulos G.N."xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/author | "Hatzopoulos G.N."xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/name | "Structure"xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/name | "Structure"xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/pages | "671-684.e5"xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/pages | "671-684.e5"xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/title | "Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel."xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/title | "Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel."xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/volume | "30"xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://purl.uniprot.org/core/volume | "30"xsd:string |
| http://purl.uniprot.org/citations/35240058 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35240058 |
| http://purl.uniprot.org/citations/35240058 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35240058 |