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http://purl.uniprot.org/citations/35247020http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/35247020http://www.w3.org/2000/01/rdf-schema#comment"

Background

Alternative splicing provides a broad strategy to amplify the genome. Yet how alternative splicing influences neurodevelopment or indeed which variants are translated at developmental choice points remains poorly explored. Here we focused on a gene important for neurodevelopment, the Lim homeodomain transcription factor, Lhx9. Lhx9 has two noncanonical splice variants, Lhx9a and Lhx9b which compared with the canonical variant Lhx9c have a truncated homeodomain and an alternative C-terminal sequence, suggesting that, if translated, these variants could differently impact on cellular function.

Results

We created a unique antibody tool designed to selectively detect noncanonical Lhx9 variants (Lhx9ab) and used this to examine the protein expression dynamics in embryos. Lhx9ab variants were translated and dynamically expressed similarly between mouse and chicken at key developmental choice points in the spinal cord, limbs and urogenital ridge. Within the spinal cord, enrichment of Lhx9c vs Lhx9ab expression was observed during key migration and axonal projection choice points.

Conclusions

These data support the notion that the expression dynamics between canonical and noncanonical Lhx9 variants could play an important role in spinal neuron maturation. More broadly, determining the temporal dynamics of alternative protein variants is a key entry point to understand how splicing influences developmental processes."xsd:string
http://purl.uniprot.org/citations/35247020http://purl.org/dc/terms/identifier"doi:10.1002/dvdy.466"xsd:string
http://purl.uniprot.org/citations/35247020http://purl.uniprot.org/core/author"Wilson S.I."xsd:string
http://purl.uniprot.org/citations/35247020http://purl.uniprot.org/core/author"Wheaton B.J."xsd:string
http://purl.uniprot.org/citations/35247020http://purl.uniprot.org/core/author"Muppavarapu M."xsd:string
http://purl.uniprot.org/citations/35247020http://purl.uniprot.org/core/author"Gonzalez-Castrillon L.M."xsd:string
http://purl.uniprot.org/citations/35247020http://purl.uniprot.org/core/author"Haggstrom S.L."xsd:string
http://purl.uniprot.org/citations/35247020http://purl.uniprot.org/core/date"2022"xsd:gYear
http://purl.uniprot.org/citations/35247020http://purl.uniprot.org/core/name"Dev Dyn"xsd:string
http://purl.uniprot.org/citations/35247020http://purl.uniprot.org/core/pages"1223-1243"xsd:string
http://purl.uniprot.org/citations/35247020http://purl.uniprot.org/core/title"Alternative LIM homeodomain splice variants are dynamically regulated at key developmental steps in vertebrates."xsd:string
http://purl.uniprot.org/citations/35247020http://purl.uniprot.org/core/volume"251"xsd:string
http://purl.uniprot.org/citations/35247020http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/35247020
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