http://purl.uniprot.org/citations/3533531 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/3533531 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/3533531 | http://www.w3.org/2000/01/rdf-schema#comment | "We have isolated cDNA clones for the human antileukoprotease HUSI-I, an elastase inhibitor, from a library, containing cDNA inserts made from human cervix uterus. A library of 10 000 recombinants was screened using a mixture of 16 different oligodeoxyribonucleotides which correspond to amino acids 79-84 and one 20mer oligodeoxyribonucleotide corresponding to amino acids 19-26. Two overlapping cDNA clones, containing the entire coding sequence and part of the 5'- and 3'-untranslated region, were isolated. DNA sequence data showed that our clone corresponds with the available protein sequence data. For expression, the cDNA fragment was inserted in a derivative of plasmid pPLc236 and expressed under the control of lambda PL promoter. Expression of antileukoprotease was proven by Western blot analysis and inhibition of chymotrypsin."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1986.tb09940.x"xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1986.tb09940.x"xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Fritz H."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Fritz H."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Appelhans H."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Appelhans H."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Machleidt W."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Machleidt W."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Seemueller U."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Seemueller U."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Gassen G."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Gassen G."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Heinzel R."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Heinzel R."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Steffens G."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/author | "Steffens G."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/date | "1986"xsd:gYear |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/pages | "61-67"xsd:string |
http://purl.uniprot.org/citations/3533531 | http://purl.uniprot.org/core/pages | "61-67"xsd:string |