| http://purl.uniprot.org/citations/35385646 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35385646 | http://www.w3.org/2000/01/rdf-schema#comment | "Ubiquitin (Ub)-binding domains embedded in intracellular proteins act as readers of the complex Ub code and contribute to regulation of numerous eukaryotic processes. Ub-interacting motifs (UIMs) are short α-helical modular recognition elements whose role in controlling proteostasis and signal transduction has been poorly investigated. Moreover, impaired or aberrant activity of UIM-containing proteins has been implicated in numerous diseases, but targeting modular recognition elements in proteins remains a major challenge. To overcome this limitation, we developed Ub variants (UbVs) that bind to 42 UIMs in the human proteome with high affinity and specificity. Structural analysis of a UbV:UIM complex revealed the molecular determinants of enhanced affinity and specificity. Furthermore, we showed that a UbV targeting a UIM in the cancer-associated Ub-specific protease 28 potently inhibited catalytic activity. Our work demonstrates the versatility of UbVs to target short α-helical Ub receptors with high affinity and specificity. Moreover, the UbVs provide a toolkit to investigate the role of UIMs in regulating and transducing Ub signals and establish a general strategy for the systematic development of probes for Ub-binding domains."xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.org/dc/terms/identifier | "doi:10.1021/acschembio.2c00089"xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/author | "Kurinov I."xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/author | "Singer A.U."xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/author | "Sicheri F."xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/author | "Sidhu S.S."xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/author | "Manczyk N."xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/author | "Veggiani G."xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/author | "Yates B.P."xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/author | "Martyn G.D."xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/name | "ACS Chem Biol"xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/pages | "941-956"xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/title | "Panel of Engineered Ubiquitin Variants Targeting the Family of Human Ubiquitin Interacting Motifs."xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/35385646 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35385646 |
| http://purl.uniprot.org/citations/35385646 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/35385646 |
| http://purl.uniprot.org/uniprot/#_P0CG47-mappedCitation-35385646 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35385646 |
| http://purl.uniprot.org/uniprot/#_Q6ZTN6-mappedCitation-35385646 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35385646 |
| http://purl.uniprot.org/uniprot/P0CG47 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35385646 |
| http://purl.uniprot.org/uniprot/Q6ZTN6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35385646 |