| http://purl.uniprot.org/citations/35451158 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/35451158 | http://www.w3.org/2000/01/rdf-schema#comment | "The endoplasmic reticulum (ER)-plasma membrane (PM) contact sites (EPCSs) are structurally conserved in eukaryotes. The Arabidopsis ER-anchored synaptotagmin 1 (SYT1), enriched in EPCSs, plays a critical role in plant abiotic stress tolerance. It has become clear that SYT1 interacts with PM to mediate ER-PM connectivity. However, whether SYT1 performs additional functions at EPCSs remains unknown. Here, we report that SYT1 efficiently transfers phospholipids between membranes. The lipid transfer activity of SYT1 is highly dependent on phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2 ], a signal lipid accumulated at the PM under abiotic stress. Mechanically, while SYT1 transfers lipids fundamentally through the synaptotagmin-like mitochondrial-lipid-binding protein (SMP) domain, the efficient lipid transport requires the C2A domain-mediated membrane tethering. Interestingly, we observed that Ca2+ could stimulate SYT1-mediated lipid transport. In addition to PI(4,5)P2 , the Ca2+ activation requires the phosphatidylserine, another negatively charged lipid on the opposed membrane. Together, our studies identified Arabidopsis SYT1 as a lipid transfer protein at EPCSs and demonstrated that it takes conserved as well as divergent mechanisms with other extend-synaptotagmins. The critical role of lipid composition and Ca2+ reveals that SYT1-mediated lipid transport is highly regulated by signals in response to abiotic stresses."xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.org/dc/terms/identifier | "doi:10.1111/tra.12844"xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/author | "Li C."xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/author | "Liu F."xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/author | "Yu H."xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/author | "Xu K."xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/author | "Wan C."xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/author | "Qian T."xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/date | "2022"xsd:gYear |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/name | "Traffic"xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/pages | "346-356"xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/title | "Arabidopsis synaptotagmin 1 mediates lipid transport in a lipid composition-dependent manner."xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/35451158 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/35451158 |
| http://purl.uniprot.org/citations/35451158 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/35451158 |
| http://purl.uniprot.org/uniprot/#_F4IFM6-mappedCitation-35451158 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35451158 |
| http://purl.uniprot.org/uniprot/#_F4IFM7-mappedCitation-35451158 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35451158 |
| http://purl.uniprot.org/uniprot/#_O04630-mappedCitation-35451158 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35451158 |
| http://purl.uniprot.org/uniprot/#_Q9SKR2-mappedCitation-35451158 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/35451158 |
| http://purl.uniprot.org/uniprot/O04630 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35451158 |
| http://purl.uniprot.org/uniprot/F4IFM6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35451158 |
| http://purl.uniprot.org/uniprot/Q9SKR2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35451158 |
| http://purl.uniprot.org/uniprot/F4IFM7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/35451158 |